Functional Viability: Measurement of Synaptic Vesicle Pool Sizes.

Autor:	Wrosch JK; Department of Psychiatry and Psychotherapy, Friedrich-Alexander University of Erlangen-Nuremberg, Schwabachanlage 6, 91054, Erlangen, Germany. Jana.Wrosch@uk-erlangen.de., Groemer TW; Department of Psychiatry and Psychotherapy, Friedrich-Alexander University of Erlangen-Nuremberg, Schwabachanlage 6, 91054, Erlangen, Germany.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2017; Vol. 1601, pp. 195-204.
DOI:	10.1007/978-1-4939-6960-9_15
Abstrakt:	Neurons and their function of conveying information across a chemical synapse are highly regulated systems. Impacts on their functional viability can occur independently from changes in morphology. Here we describe a method to assess the size of synaptic vesicle pools using live cell fluorescence imaging and a genetically encoded probe (pHluorin). Assessing functional parameters such as the size of synaptic vesicle pools can be a valuable addition to common assays of neuronal cell viability as they demonstrate that key cellular functions are intact.
Databáze:	MEDLINE
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