Assaying Spontaneous Network Activity and Cellular Viability Using Multi-well Microelectrode Arrays.

Autor:	Brown JP; Integrated Systems Toxicology Division, NHEERL, US EPA, MD105-05, Research Triangle Park, NC, 27711, USA., Lynch BS; Integrated Systems Toxicology Division, NHEERL, US EPA, MD105-05, Research Triangle Park, NC, 27711, USA., Curry-Chisolm IM; Integrated Systems Toxicology Division, NHEERL, US EPA, MD105-05, Research Triangle Park, NC, 27711, USA., Shafer TJ; Integrated Systems Toxicology Division, NHEERL, US EPA, MD105-05, Research Triangle Park, NC, 27711, USA. shafer.tim@epa.gov., Strickland JD; Axion Biosystems, Atlanta, GA, USA.; Department of Pharmacology and Toxicology, Michigan State University, E. Lansing, MI, USA.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2017; Vol. 1601, pp. 153-170.
DOI:	10.1007/978-1-4939-6960-9_13
Abstrakt:	Microelectrode array (MEA) technology is a neurophysiological method that allows for the spontaneous measure of activity in neural cultures and determination of drug and chemical effects thereon. Recent introduction of multi-well MEA (mwMEA) formats have dramatically increased the throughput of this technology, allowing more efficient compound screening. Rapid characterization of compounds for neuroactivity or neurotoxicity hazard evaluation following acute, chronic, or developmental exposures ideally would also consider compound effects on cell health, and to do so in the same well requires a multiplexed approach. Procedures describing the multiplexed method to acute and developmental screening are described in this chapter.
Databáze:	MEDLINE
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