Time Course Expression Analysis of 1[2-cyano-3,12-dioxooleana-1,9(11)-dien-28-oyl]imidazole Induction of Cytoprotection in Human Endothelial Cells.

Autor: Bynum JA; U.S. Army Institute of Surgical Research, San Antonio, TX, USA.; Division of Pharmaceutics, College of Pharmacy, The University of Texas at Austin, Austin, TX, USA., Wang X; Department of Pharmaceutical Sciences, School of Pharmacy, Philadelphia College of Osteopathic Medicine-Georgia Campus, Suwanee, GA, USA., Stavchansky SA; Division of Pharmaceutics, College of Pharmacy, The University of Texas at Austin, Austin, TX, USA., Bowman PD; U.S. Army Institute of Surgical Research, San Antonio, TX, USA.
Jazyk: angličtina
Zdroj: Gene regulation and systems biology [Gene Regul Syst Bio] 2017 Apr 07; Vol. 11, pp. 1177625017701106. Date of Electronic Publication: 2017 Apr 07 (Print Publication: 2017).
DOI: 10.1177/1177625017701106
Abstrakt: 1[2-cyano-3,12-dioxooleana-1,9(11)-dien-28-oyl]imidazole (CDDO-Im), a synthetic derivative of oleanolic acid that exhibits antioxidant and anti-inflammatory activity in several animal and in vitro models, has been shown to be beneficial if given after injury. Although induction of heme oxygenase 1 appears to be a major effector of cytoprotection, the mechanism by which the overall effect is mediated is largely unknown. This study evaluated temporal gene expression profiles to better characterize the early transcriptional events and their relationship to the dynamics of the cytoprotective response in human umbilical vein endothelial cells (HUVEC) to CDDO-Im. Time-course gene expression profiling was performed on HUVEC treated with CDDO-Im for 0.5, 1, 3, 6, and 24 hours. More than 10 000 genes were statistically altered in their expression in at least 1 time point across the time course. Large alterations in immediate-early gene expression were readily detectable within 0.5 hour after administration of CDDO-Im.
Competing Interests: DECLARATION OF CONFLICTING INTERESTS: The author(s) declared the following potential conflicts of interest with respect to the research, authorship, and/or publication of this article: The opinions or assertions contained herein are the private views of the author and are not to be construed as official or as reflecting the views of the Department of the Army or the Department of Defense.
Databáze: MEDLINE
Externí odkaz: