In vitro fibrillogenesis of tropocollagen type III in collagen type I affects its relative fibrillar topology and mechanics.

Autor:	Asgari M; Department of Mechanical Engineering, McGill University, 817 Sherbrooke Street West, Montreal, QC, H3A 0C3, Canada. meisam.asgari@mail.mcgill.ca., Latifi N; Department of Mechanical Engineering, McGill University, 817 Sherbrooke Street West, Montreal, QC, H3A 0C3, Canada., Heris HK; Department of Bioengineering, McGill University, 817 Sherbrooke Street West, Montreal, QC, H3A 0C3, Canada., Vali H; Department of Anatomy & Cell Biology, McGill University, 3640 University Street, Montreal, QC, H3A 2B2, Canada., Mongeau L; Department of Mechanical Engineering, McGill University, 817 Sherbrooke Street West, Montreal, QC, H3A 0C3, Canada.
Jazyk:	angličtina
Zdroj:	Scientific reports [Sci Rep] 2017 May 03; Vol. 7 (1), pp. 1392. Date of Electronic Publication: 2017 May 03.
DOI:	10.1038/s41598-017-01476-y
Abstrakt:	Tropocollagen types I and III were simultaneously fibrilized in vitro, and the differences between the geometric and mechanical properties of the heterotypic fibrils with different mixing ratios of tropocollagen III to I were investigated. Transmission electron microscopy was used to confirm the simultaneous presence of both tropocollagen types within the heterotypic fibrils. The incorporation of collagen III in I caused the fibrils to be thinner with a shorter D-banding than pure collagen I. Hertzian contact model was used to obtain the elastic moduli from atomic force microscope indentation testing using a force volume analysis. The results indicated that an increase in the percentage of tropocollagen III reduced the mechanical stiffness of the obtained fibrils. The mechanical stiffness of the collagen fibrils was found to be greater at higher loading frequencies. This observation might explain the dominance of collagen III over I in soft distensible organs such as human vocal folds.
Databáze:	MEDLINE
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