Characterization of genomic changes in the cervical pre-cancerous lesions and tumors induced by different types of human papillomaviruses.

Autor: Grozdanov P; The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 26 Georgi Bonchev, 1113 Sofia, Bulgaria., Hadjidekova S; Department of Medical Genetics, Medical University, Sofia, Bulgaria., Dimova I; Department of Medical Genetics, Medical University, Sofia, Bulgaria., Nikolova I; The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 26 Georgi Bonchev, 1113 Sofia, Bulgaria., Toncheva D; Department of Medical Genetics, Medical University, Sofia, Bulgaria., Ganchev G; Specialized Hospital for Active Treatment in Oncology, Sofia, Bulgaria., Zlatkov V; The University Obstetrics and Gynecology Hospital 'Maichin Dom', Sofia, Bulgaria., Galabov AS; The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 26 Georgi Bonchev, 1113 Sofia, Bulgaria.
Jazyk: angličtina
Zdroj: Virusdisease [Virusdisease] 2016 Sep; Vol. 27 (3), pp. 271-276. Date of Electronic Publication: 2016 Aug 09.
DOI: 10.1007/s13337-016-0338-4
Abstrakt: Cervical carcinoma is the second most common malignancy among women in both incidence and mortality. Although much is known about the etiology and treatment of cervical cancer, the role of genetic alterations in the multistep pathway of cervical tumorigenesis is largely unknown. The aim of this study was to characterize the genomic changes in the cervical pre-cancerous lesions and tumors, induced by different types of human papillomaviruses. In this research was used the BlueGnome CytoChip oligo 2 × 105 K microarray for whole-genome oligo-array CGH. Microarray CGH analysis of 40 specimens was carried out-12 specimens from patients with early-stage squamous cell carcinomas; 19 specimens from patients with mild to moderate dysplasia and 9 with severe dysplasia. First we performed microarray CGH analysis of five DNA pools which contained the DNA from homogeneous groups of patients. The results revealed presence of micro chromosomal aberrations in chromosome region 14q11.2. According to the genome database these aberrations represent polymorphisms. Microarray analysis of DNA from 9 separate carcinoma lesions revealed a total of 26 aberrations in 14 chromosomes of nine patients. Our results showed the advantages of high-resolution chips in the clinical diagnosis of patients with cancerous and precancerous lesions caused by viral infection with HPV, but also highlight the need for extensive population studies revealing the molecular nature and clinical significance of different CNVs and the creation of detailed maps of variations in the Bulgarian population. This would facilitate extremely precise interpretation of specific genomic imbalances in the clinical aspect.
Databáze: MEDLINE
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