Enhanced therapeutic benefit of quercetin-loaded phytosome nanoparticles in ovariectomized rats.

Autor: Abd El-Fattah AI; Biochemistry Department, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo, Egypt. Electronic address: abeeribrahim75@yahoo.com., Fathy MM; Biophysics Department, Faculty of Science, Cairo University, 12613 Giza, Egypt. Electronic address: mfathy@sci.cu.edu.eg., Ali ZY; National Organization of Drug Control and Research (NODCAR), 6 Abou Hazem St., Madkour Station, Alharm St., 12553 Giza, Egypt. Electronic address: zeinabyousef65@ymail.com., El-Garawany AEA; National Organization of Drug Control and Research (NODCAR), 6 Abou Hazem St., Madkour Station, Alharm St., 12553 Giza, Egypt. Electronic address: AbdElRahmanElGarawany@hotmail.com., Mohamed EK; National Organization of Drug Control and Research (NODCAR), 6 Abou Hazem St., Madkour Station, Alharm St., 12553 Giza, Egypt. Electronic address: drehsankhedre@hotmail.com.
Jazyk: angličtina
Zdroj: Chemico-biological interactions [Chem Biol Interact] 2017 Jun 01; Vol. 271, pp. 30-38. Date of Electronic Publication: 2017 Apr 29.
DOI: 10.1016/j.cbi.2017.04.026
Abstrakt: Quercetin, a dietary flavonol phytoestrogen, has many health benefits but it is poorly absorbed when administered orally. To improve its bioavailability, we prepared quercetin-loaded phytosome nanoparticles (QP) using the thin film hydration method. The prepared nano-formulations were characterized using different techniques. Transmission electron microscopy revealed the homogeneously spherical, well and uniformly dispersed, nano-sized nature of QP. Dynamic light scattering measurements of QP (70 ± 7.44 nm) also confirmed this. Stability of the formed nanoparticles was established via zeta potential determination. The prepared QP exhibited very high encapsulation efficiency (98.4%). The estrogenic activity of QP, concerning inflammation, oxidative stress, bone, lipid profile, blood glucose level and weight gain, was investigated in ovariectomized rat model using 10 and 50 mg/kg/day oral doses for 4 weeks. Treatment with QP showed significant increase in serum calcium, inorganic phosphorus and glutathione content. Whereas, it significantly decreased serum alkaline phosphatase, acid phosphatase, malondialdehyde level, tumor necrosis factor-alpha and glucose level and improved lipid profile. Consequently, the results obtained confirm the superiority of QP over free quercetin at the same doses as a promising hormone replacement therapy.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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