Influence of static magnetic field exposure on fatty acid composition in Salmonella Hadar.

Autor: Ben Mouhoub R; Biochemistry and Molecular Biology, Code UR13ES34 Research Unit, Faculty of Sciences of Bizerte, Zarzouna 7021, Carthage University, Tunisia. Electronic address: ramlabenmouhoub@gmail.com., El May A; Biochemistry and Molecular Biology, Code UR13ES34 Research Unit, Faculty of Sciences of Bizerte, Zarzouna 7021, Carthage University, Tunisia., Cheraief I; Laboratory of Biochemistry, UR: ''Human Nutrition and Metabolic Disorder', Faculty of Medicine, Monastir 5019, Tunisia., Landoulsi A; Biochemistry and Molecular Biology, Code UR13ES34 Research Unit, Faculty of Sciences of Bizerte, Zarzouna 7021, Carthage University, Tunisia.
Jazyk: angličtina
Zdroj: Microbial pathogenesis [Microb Pathog] 2017 Jul; Vol. 108, pp. 13-20. Date of Electronic Publication: 2017 Apr 25.
DOI: 10.1016/j.micpath.2017.04.035
Abstrakt: We have been interested, in this work, to investigate the effect of the exposure to static magnetic field at 200 mT (SMF) on the fatty acid (FA) composition of Salmonella enterica subsp Enterica serovar Hadar isolate 287: effects on the proportion of saturated and unsaturated fatty acids (SFAs, UFAs), cyclopropane fatty acids (CFAs) and hydroxy fatty acids after exposure to the static magnetic field at 200 mT (SMF). Analysis with Gas Chromatography-Mass Spectrometry (GC-MS) of total lipid showed that the proportion of the most fatty acids was clearly affected. The comparison of UFAs/SFAs ratio in exposed bacteria and controls showed a diminution after 3 and 6 h of exposure. This ration reached a balance after 9 h of treatment with SMF. So we can conclude that S. Hadar tries to adapt to magnetic stress by changing the proportions of SFAs and UFAs over time to maintain an equilibrium after 9 h of exposure, thus to maintain the inner membranes fluidity. Also, a decrease in the proportion of hydroxy FAs was observed after 6 h but an increase of this proportion after 9 h of exposure. Concerning CFAs, its proportion raised after 6 h of exposure to the SMF but it decreased after 9 h of exposure. These results are strongly correlated with those of cfa (cyclopropane fatty acid synthase) gene expression which showed a decrease of its expression after 9 h of exposure.
(Copyright © 2017 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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