| Autor: |
Siva Sankar P; School of Biological Sciences, Universiti Sains Malaysia, 11800 Gelugor, Malaysia.; Infectomics Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, 13200 Kepala Batas, Malaysia., Che Mat MF; Molecular Pathology Unit, Cancer Research Centre, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia., Muniandy K; Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800 Gelugor, Malaysia., Xiang BLS; School of Biological Sciences, Universiti Sains Malaysia, 11800 Gelugor, Malaysia., Ling PS; School of Biological Sciences, Universiti Sains Malaysia, 11800 Gelugor, Malaysia., Hoe SLL; Molecular Pathology Unit, Cancer Research Centre, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia., Khoo AS; Molecular Pathology Unit, Cancer Research Centre, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia., Mohana-Kumaran N; School of Biological Sciences, Universiti Sains Malaysia, 11800 Gelugor, Malaysia. |
| Abstrakt: |
Nasopharyngeal carcinoma (NPC) is a type of cancer endemic in Asia, including Malaysia, Southern China, Hong Kong and Taiwan. Treatment resistance, particularly in recurring cases, remains a challenge. Thus, studies to develop novel therapeutic agents are important. Potential therapeutic compounds may be effectively examined using two-dimensional (2D) cell culture models, three-dimensional (3D) spheroid models or in vivo animal models. The majority of drug assessments for cancers, including for NPC, are currently performed with 2D cell culture models. This model offers economical and high-throughput screening advantages. However, 2D cell culture models cannot recapitulate the architecture and the microenvironment of a tumor. In vivo models may recapitulate certain architectural and microenvironmental conditions of a tumor, however, these are not feasible for the screening of large numbers of compounds. By contrast, 3D spheroid models may be able to recapitulate a physiological microenvironment not observed in 2D cell culture models, in addition to avoiding the impediments of in vivo animal models. Thus, the 3D spheroid model offers a more representative model for the study of NPC growth, invasion and drug response, which may be cost-effective without forgoing quality. |
