The MBD7 complex promotes expression of methylated transgenes without significantly altering their methylation status.

Autor: Li D; Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California, Riverside, United States.; School of Life Sciences, Lanzhou University, Lanzhou, China.; State Key Laboratory of Plant Cell and Chromosome Engineering, Hebei Collaboration Innovation Center for Cell Signaling, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, China., Palanca AMS; Plant Molecular and Cellular Biology Laboratory, Salk Institute for Biological Studies, La Jolla, United States., Won SY; Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California, Riverside, United States., Gao L; Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California, Riverside, United States.; College of Life Sciences and Oceanography, Guangdong Provincial Key Laboratory for Plant Epigenetics, Shenzhen University, Shenzhen, China., Feng Y; Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California, Riverside, United States.; State Key Laboratory of Protein and Plant Gene research, College of Life Sciences, Peking University, Beijing, China., Vashisht AA; Department of Biological Chemistry, David Geffen School of Medicine at UCLA, Los Angeles, United States., Liu L; Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California, Riverside, United States., Zhao Y; Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California, Riverside, United States., Liu X; Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California, Riverside, United States.; State Key Laboratory of Plant Cell and Chromosome Engineering, Hebei Collaboration Innovation Center for Cell Signaling, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, China., Wu X; Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California, Riverside, United States.; Laboratory of Molecular Biology and Protein Science, Laboratory of the Ministry of Education, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, China., Li S; Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California, Riverside, United States., Le B; Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California, Riverside, United States., Kim YJ; Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California, Riverside, United States., Yang G; Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California, Riverside, United States., Li S; Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California, Riverside, United States., Liu J; Laboratory of Molecular Biology and Protein Science, Laboratory of the Ministry of Education, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, China., Wohlschlegel JA; Department of Biological Chemistry, David Geffen School of Medicine at UCLA, Los Angeles, United States., Guo H; State Key Laboratory of Protein and Plant Gene research, College of Life Sciences, Peking University, Beijing, China., Mo B; College of Life Sciences and Oceanography, Guangdong Provincial Key Laboratory for Plant Epigenetics, Shenzhen University, Shenzhen, China., Chen X; Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California, Riverside, United States.; Howard Hughes Medical Institute, University of California, Riverside, United States., Law JA; Plant Molecular and Cellular Biology Laboratory, Salk Institute for Biological Studies, La Jolla, United States.
Jazyk: angličtina
Zdroj: ELife [Elife] 2017 Apr 28; Vol. 6. Date of Electronic Publication: 2017 Apr 28.
DOI: 10.7554/eLife.19893
Abstrakt: DNA methylation is associated with gene silencing in eukaryotic organisms. Although pathways controlling the establishment, maintenance and removal of DNA methylation are known, relatively little is understood about how DNA methylation influences gene expression. Here we identified a METHYL-CpG-BINDING DOMAIN 7 (MBD7) complex in Arabidopsis thaliana that suppresses the transcriptional silencing of two LUCIFERASE ( LUC) reporters via a mechanism that is largely downstream of DNA methylation. Although mutations in components of the MBD7 complex resulted in modest increases in DNA methylation concomitant with decreased LUC expression, we found that these hyper-methylation and gene expression phenotypes can be genetically uncoupled. This finding, along with genome-wide profiling experiments showing minimal changes in DNA methylation upon disruption of the MBD7 complex, places the MBD7 complex amongst a small number of factors acting downstream of DNA methylation. This complex, however, is unique as it functions to suppress, rather than enforce, DNA methylation-mediated gene silencing.
Databáze: MEDLINE