Perturbation of DROSHA and DICER expression by human papillomavirus 16 oncoproteins.

Autor: Harden ME; Program in Virology, Division of Medical Sciences, Harvard Medical School Boston, MA 02115, USA; Department of Developmental, Molecular and Chemical Biology, Tufts University School of Medicine, Boston, MA 02111, USA., Munger K; Department of Developmental, Molecular and Chemical Biology, Tufts University School of Medicine, Boston, MA 02111, USA. Electronic address: karl.munger@tufts.edu.
Jazyk: angličtina
Zdroj: Virology [Virology] 2017 Jul; Vol. 507, pp. 192-198. Date of Electronic Publication: 2017 Apr 24.
DOI: 10.1016/j.virol.2017.04.022
Abstrakt: Many tumors, including cervical carcinoma, show dysregulated expression of the microRNA processing machinery, specifically DROSHA and DICER. Some cervical cancers exhibit chromosome 5p amplifications and DROSHA is the most significantly upregulated transcript and is observed in all tumors with 5p gain. DROSHA and DICER mRNA levels, however, are higher in HPV positive cancer lines than in an HPV negative cervical carcinoma line. We show that high-risk HPV E6/E7 expression in HPV negative C33A cervical carcinoma cells and primary human epithelial cell causes increased expression of DROSHA and DICER mRNA and protein. Most importantly, many DROSHA regulated microRNAs are dysregulated in HPV16 E6/E7 expressing cells. These results suggest that increased DROSHA levels contribute to HPV16 E6/E7 dysregulation of cellular microRNA expression.
(Copyright © 2017 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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