Manufacturing Differences Affect Human Bone Marrow Stromal Cell Characteristics and Function: Comparison of Production Methods and Products from Multiple Centers.

Autor: Liu S; Cell Processing Section, Department of Transfusion Medicine, Clinical Center; National Institutes of Health, Bethesda, Maryland, USA., de Castro LF; Skeletal Biology Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland, USA., Jin P; Cell Processing Section, Department of Transfusion Medicine, Clinical Center; National Institutes of Health, Bethesda, Maryland, USA., Civini S; Cell Processing Section, Department of Transfusion Medicine, Clinical Center; National Institutes of Health, Bethesda, Maryland, USA., Ren J; Cell Processing Section, Department of Transfusion Medicine, Clinical Center; National Institutes of Health, Bethesda, Maryland, USA., Reems JA; Cell Therapy and Regenerative Medicine, University of Utah, Salt Lake City, Utah, USA.; Biomedical Excellence for Safer Transfusion (BEST) group, Darthmouth-Hitchcock Medical Center, One Medical Center Drive, Lebanon, New Hamphire 03756, USA., Cancelas J; Biomedical Excellence for Safer Transfusion (BEST) group, Darthmouth-Hitchcock Medical Center, One Medical Center Drive, Lebanon, New Hamphire 03756, USA.; Hoxworth Blood Center &Department of Pediatrics, The University of Cincinnati, Cincinnati, Ohio, USA., Nayak R; Hoxworth Blood Center &Department of Pediatrics, The University of Cincinnati, Cincinnati, Ohio, USA., Shaw G; Regenerative Medicine Institute, National Centre for Biomedical Engineering and Science, National University of Ireland Galway, Galway, Ireland., O'Brien T; Biomedical Excellence for Safer Transfusion (BEST) group, Darthmouth-Hitchcock Medical Center, One Medical Center Drive, Lebanon, New Hamphire 03756, USA.; Regenerative Medicine Institute, National Centre for Biomedical Engineering and Science, National University of Ireland Galway, Galway, Ireland., McKenna DH; Biomedical Excellence for Safer Transfusion (BEST) group, Darthmouth-Hitchcock Medical Center, One Medical Center Drive, Lebanon, New Hamphire 03756, USA.; Molecular and Cellular Therapeutics, University of Minnesota, Minneapolis, Minnesota, USA.; Production Assistance for Cell Therapies (PACT) group, National Heart, Lung and Blood Program, PACT Coordinating Center, The Emmes Corporation, 401 North Washington Street, Suite 700, Rockville, Maryland 20850, USA., Armant M; Production Assistance for Cell Therapies (PACT) group, National Heart, Lung and Blood Program, PACT Coordinating Center, The Emmes Corporation, 401 North Washington Street, Suite 700, Rockville, Maryland 20850, USA.; Center for Human Cell Therapy, Programs in Cellular &Molecular Medicine, Boston Children's Hospital, Boston, Massachusetts, USA., Silberstein L; Production Assistance for Cell Therapies (PACT) group, National Heart, Lung and Blood Program, PACT Coordinating Center, The Emmes Corporation, 401 North Washington Street, Suite 700, Rockville, Maryland 20850, USA.; Center for Human Cell Therapy, Programs in Cellular &Molecular Medicine, Boston Children's Hospital, Boston, Massachusetts, USA., Gee AP; Production Assistance for Cell Therapies (PACT) group, National Heart, Lung and Blood Program, PACT Coordinating Center, The Emmes Corporation, 401 North Washington Street, Suite 700, Rockville, Maryland 20850, USA.; Center for Cell and Gene Therapy, Texas Children's Hospital, The Methodist Hospital, and Baylor College of Medicine, Houston, Texas, USA., Hei DJ; Production Assistance for Cell Therapies (PACT) group, National Heart, Lung and Blood Program, PACT Coordinating Center, The Emmes Corporation, 401 North Washington Street, Suite 700, Rockville, Maryland 20850, USA.; Waisman Biomanufacturing, University of Wisconsin-Madison, Madison, Wisconsin, USA., Hematti P; Production Assistance for Cell Therapies (PACT) group, National Heart, Lung and Blood Program, PACT Coordinating Center, The Emmes Corporation, 401 North Washington Street, Suite 700, Rockville, Maryland 20850, USA.; Department of Medicine, University of Wisconsin-Madison, Madison, Wisconsin, USA.; University of Wisconsin Carbone Cancer Center, Madison, Wisconsin, USA., Kuznetsov SA; Skeletal Biology Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland, USA., Robey PG; Skeletal Biology Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland, USA., Stroncek DF; Cell Processing Section, Department of Transfusion Medicine, Clinical Center; National Institutes of Health, Bethesda, Maryland, USA.; Biomedical Excellence for Safer Transfusion (BEST) group, Darthmouth-Hitchcock Medical Center, One Medical Center Drive, Lebanon, New Hamphire 03756, USA.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2017 Apr 27; Vol. 7, pp. 46731. Date of Electronic Publication: 2017 Apr 27.
DOI: 10.1038/srep46731
Abstrakt: Human bone marrow stromal cells (BMSCs, also known as bone marrow-derived mesenchymal stem cells) are manufactured using many different methods, but little is known about the spectrum of manufacturing methods used and their effects on BMSC characteristics and function. Seven centers using, and one developing, Good Manufacturing Practices (GMP) processes were surveyed as to their production methods. Among the seven centers, all used marrow aspirates as the starting material, but no two centers used the same manufacturing methods. Two to four BMSC lots from each center were compared using global gene expression. Among the twenty-four BMSC lots from the eight centers intra-center transcriptome variability was low and similar among centers. Principal component analysis and unsupervised hierarchical clustering analysis separated all the lots from five centers into five distinct clusters. BMSCs from six of the eight centers were tested for their ability to form bone and support hematopoiesis by in vivo transplantation (defining features of BMSCs). Those from all six centers tested formed bone, but the quantity formed was highly variable and BMSCs from only three centers supported hematopoiesis. These results show that differences in manufacturing resulted in variable BMSC characteristics including their ability to form bone and support hematopoiesis.
Databáze: MEDLINE