Morphometric developmental pattern of bovine handmade cloned concepti in late pregnancy.

Autor:	Gerger RPC; Center of Agronomy and Veterinary Sciences, Santa Catarina State University (CAV/UDESC), Av. Luiz de Camões, 2090, Bairro Conta Dinheiro, Lages, SC 88.520-000, Brazil., Zago FC; Santa Catarina State Agriculture Research and Extension Center (EPAGRI), Rua João José Godinho, S/N, PO Box 181, Bairro Morro do Posto, Lages, SC 88.502-970, Brazil., Ribeiro ES; Center of Agronomy and Veterinary Sciences, Santa Catarina State University (CAV/UDESC), Av. Luiz de Camões, 2090, Bairro Conta Dinheiro, Lages, SC 88.520-000, Brazil., Gaudencio Neto S; Center of Agronomy and Veterinary Sciences, Santa Catarina State University (CAV/UDESC), Av. Luiz de Camões, 2090, Bairro Conta Dinheiro, Lages, SC 88.520-000, Brazil., Martins LT; Center of Agronomy and Veterinary Sciences, Santa Catarina State University (CAV/UDESC), Av. Luiz de Camões, 2090, Bairro Conta Dinheiro, Lages, SC 88.520-000, Brazil., Aguiar LH; Center of Agronomy and Veterinary Sciences, Santa Catarina State University (CAV/UDESC), Av. Luiz de Camões, 2090, Bairro Conta Dinheiro, Lages, SC 88.520-000, Brazil., Rodrigues VHV; Molecular and Developmental Biology Laboratory, University of Fortaleza (UNIFOR), Av. Washington Soares, n.1321, Bairro Edson Queiroz. Fortaleza, CE 60.811-905, Brazil., Furlan FH; Center of Agronomy and Veterinary Sciences, Santa Catarina State University (CAV/UDESC), Av. Luiz de Camões, 2090, Bairro Conta Dinheiro, Lages, SC 88.520-000, Brazil., Ortigari I; Center of Agronomy and Veterinary Sciences, Santa Catarina State University (CAV/UDESC), Av. Luiz de Camões, 2090, Bairro Conta Dinheiro, Lages, SC 88.520-000, Brazil., Sainz RD; Department of Animal Science, University of California, Davis (UC Davis), CA 95616-8521, USA., Ferrell CL; US Meat Animal Research Center, ARS, USDA, Clay Center, NE 68933, USA., Miglino MA; School of Veterinary Medicine and Animal Science, University of Sao Paulo (USP), Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo, SP 05.508-270, Brazil., Ambrósio CE; School of Veterinary Medicine and Animal Science, University of Sao Paulo (USP), Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo, SP 05.508-270, Brazil., Rodrigues JL; School of Veterinary Medicine (FAVET), Federal University of Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, 9090, Bairro Agronomia, Porto Alegre, RS 91540-000, Brazil., Rossetto R; Molecular and Developmental Biology Laboratory, University of Fortaleza (UNIFOR), Av. Washington Soares, n.1321, Bairro Edson Queiroz. Fortaleza, CE 60.811-905, Brazil., Forell F; Center of Agronomy and Veterinary Sciences, Santa Catarina State University (CAV/UDESC), Av. Luiz de Camões, 2090, Bairro Conta Dinheiro, Lages, SC 88.520-000, Brazil., Bertolini LR; Molecular and Developmental Biology Laboratory, University of Fortaleza (UNIFOR), Av. Washington Soares, n.1321, Bairro Edson Queiroz. Fortaleza, CE 60.811-905, Brazil., Bertolini M; Center of Agronomy and Veterinary Sciences, Santa Catarina State University (CAV/UDESC), Av. Luiz de Camões, 2090, Bairro Conta Dinheiro, Lages, SC 88.520-000, Brazil.
Jazyk:	angličtina
Zdroj:	Reproduction, fertility, and development [Reprod Fertil Dev] 2017 Apr; Vol. 29 (5), pp. 950-967.
DOI:	10.1071/RD15215
Abstrakt:	Cloning procedures often interfere with conceptus growth and life ex utero, in a set of symptoms known as abnormal offspring syndrome (AOS). The aim of the present study was to compare the developmental pattern of in vivo-derived (IVD), IVF-derived and handmade cloning-derived (NT-HMC) Day 225 bovine concepti using established procedures. Pregnancy diagnosis was performed on Day 30 following blastocyst transfer on Day 7. Conceptus morphometry was assessed by ultrasonography on Day 51, and on Day 225 pregnant cows were killed for morphological examination of concepti. Pregnancy outcome was similar between groups, with greater pregnancy losses in the first trimester (70.6%) and smaller fetuses on Day 51 in the NT-HMC group than in the IVD (14.3%) and IVF (20.0%) groups. However, NT-HMC-derived concepti were twofold larger on Day 225 of gestation than controls. A higher frequency (63.5%) of placentomes larger than the largest in the IVD group was observed in the NT-HMC group, which may be relevant to placental function. Conceptus traits in the IVF group were similar to the IVD controls, with only slight changes in placentome types. Morphological changes in cloned concepti likely affected placental function and metabolism, disrupting the placental constraining mechanism on fetal growth in mid- to late pregnancy.
Databáze:	MEDLINE
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