Se-SAD serial femtosecond crystallography datasets from selenobiotinyl-streptavidin.

Autor: Yoon CH; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA., DeMirci H; Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA.; Biosciences Division, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA.; Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA., Sierra RG; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA., Dao EH; Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA.; Biosciences Division, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA., Ahmadi R; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA., Aksit F; Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA., Aquila AL; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA., Batyuk A; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA., Ciftci H; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA., Guillet S; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA., Hayes MJ; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA., Hayes B; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA., Lane TJ; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA.; Biosciences Division, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA., Liang M; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA., Lundström U; Biosciences Division, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA., Koglin JE; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA., Mgbam P; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA., Rao Y; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA., Rendahl T; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA., Rodriguez E; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA., Zhang L; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA., Wakatsuki S; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA.; Biosciences Division, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA., Boutet S; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA., Holton JM; Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA.; Department of Biochemistry and Biophysics, University of California, San Francisco, California 94158, USA., Hunter MS; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA.
Jazyk: angličtina
Zdroj: Scientific data [Sci Data] 2017 Apr 25; Vol. 4, pp. 170055. Date of Electronic Publication: 2017 Apr 25.
DOI: 10.1038/sdata.2017.55
Abstrakt: We provide a detailed description of selenobiotinyl-streptavidin (Se-B SA) co-crystal datasets recorded using the Coherent X-ray Imaging (CXI) instrument at the Linac Coherent Light Source (LCLS) for selenium single-wavelength anomalous diffraction (Se-SAD) structure determination. Se-B SA was chosen as the model system for its high affinity between biotin and streptavidin where the sulfur atom in the biotin molecule (C 10 H 16 N 2 O 3 S) is substituted with selenium. The dataset was collected at three different transmissions (100, 50, and 10%) using a serial sample chamber setup which allows for two sample chambers, a front chamber and a back chamber, to operate simultaneously. Diffraction patterns from Se-B SA were recorded to a resolution of 1.9 Å. The dataset is publicly available through the Coherent X-ray Imaging Data Bank (CXIDB) and also on LCLS compute nodes as a resource for research and algorithm development.
Databáze: MEDLINE
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