Serological biomarkers detect active joint destruction and inflammation in patients with haemophilic arthropathy.

Autor: Hua B; Department of Hematology, Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College (PUMC), Peking Union Medical College Hospital (PUMCH), Beijing, China., Olsen EHN; Global Research, Novo Nordisk A/S, Måløv, Denmark., Sun S; Nordic Bioscience A/S, Herlev, Denmark., Gudme CN; Global Research, Novo Nordisk A/S, Måløv, Denmark., Wang L; Novo Nordisk Research Centre China, Beijing, China., Vandahl B; Global Research, Novo Nordisk A/S, Måløv, Denmark., Roepstorff K; Global Research, Novo Nordisk A/S, Måløv, Denmark., Kjelgaard-Hansen M; Global Research, Novo Nordisk A/S, Måløv, Denmark., Sørensen BB; Global Research, Novo Nordisk A/S, Måløv, Denmark., Zhao Y; Department of Hematology, Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College (PUMC), Peking Union Medical College Hospital (PUMCH), Beijing, China., Karsdal MA; Nordic Bioscience A/S, Herlev, Denmark., Manon-Jensen T; Nordic Bioscience A/S, Herlev, Denmark.
Jazyk: angličtina
Zdroj: Haemophilia : the official journal of the World Federation of Hemophilia [Haemophilia] 2017 Jul; Vol. 23 (4), pp. e294-e300. Date of Electronic Publication: 2017 Apr 24.
DOI: 10.1111/hae.13196
Abstrakt: Introduction: Progressive arthropathy caused by recurrent joint bleeds is a severe complication in haemophilia.
Aim: We investigated whether biomarkers of cartilage and bone degradation, and inflammation were altered in haemophilia patients and whether these biomarkers could identify haemophilia patients with arthropathy.
Methods: Serum from 35 haemophilia patients with varying degrees of arthropathy and 43 age- and gender-matched control subjects were analysed. Biomarkers of cartilage degradation (C2M, COMP, CTX-II, ADAMTS5), cartilage formation (PRO-C2), bone formation (PINP), bone resorption (CTX-I) and inflammation (hsCRP, CRPM) were measured by ELISA. Arthropathy was assessed by radiological evaluation (Pettersson score) and physical examination (Gilbert score).
Results: In patients with haemophilia, cartilage degradation, measured by C2M, CTX-II and COMP, was increased by 25% (P < 0.05) compared with control subjects. Levels of the cartilage degradation enzyme, ADAMTS5, were 10% lower in haemophilia patients (P < 0.05). Bone formation (PINP) was reduced by 25% (P < 0.05) in haemophilia patients, whereas bone resorption (CTX-I) was increased by 30% (P < 0.001). Acute inflammation (hsCRP) was increased by 50% (P < 0.01), whereas chronic inflammation (CRPM) was decreased by 25% (P < 0.0001). The hsCRP/CRPM ratio was 60% higher (P < 0.001) in haemophilia patients relative to control subjects. A biomarker panel combining C2M, CRPM, and ADAMTS5 could distinguish haemophilia patients from control subjects with 85.3% accuracy (P < 0.0001). We found no strong correlation between biomarkers and radiological and physical examination of the joint.
Conclusion: Biomarkers detect increased cartilage and bone degradation, and altered inflammatory activity in haemophilia patients with arthropathy. These biomarkers could potentially be used to identify patients with progressing joint disease.
(© 2017 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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