| Autor: |
Filiault DL; Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna Biocenter (VBC), Dr. Bohr-Gasse 3, 1030, Vienna, Austria., Seymour DK; Department of Ecology and Evolutionary Biology, University of California, Irvine, Irvine, CA, USA., Maruthachalam R; School of Biology, Indian Institute of Science Education and Research (IISER), Thiruvananthapuram, Kerala, India., Maloof JN; Department of Plant Biology, University of California, Davis, 1 Shields Ave., Davis, CA, USA. jnmaloof@ucdavis.edu. |
| Abstrakt: |
Recombinant inbred lines (RILs) are an essential tool for quantitative trait locus (QTL) mapping in Arabidopsis thaliana. Conventionally, the development of these lines is a time-consuming and tedious process requiring six to eight generations of selfing. Here, we describe an alternative approach: the rapid generation of RILs in A. thaliana via the creation of doubled haploids. In this method, F 1 plants are crossed to an engineered haploid inducer to produce haploid plants. The chromosomes of these haploids then spontaneously double, generating immortalized homozygous F 2 lines called doubled haploid RILs (DH RILs). Finally, DH RILs are genotyped using low-coverage whole-genome sequencing and are ready to be used for QTL mapping. |
