Cervid herpesvirus 2 and not Moraxella bovoculi caused keratoconjunctivitis in experimentally inoculated semi-domesticated Eurasian tundra reindeer.

Autor: Tryland M; Arctic Infection Biology, Department of Arctic and Marine Biology, UiT-The Arctic University of Norway, POBox 6050, Langnes, 9037, Tromsø, Norway. morten.tryland@uit.no., Romano JS; Arctic Infection Biology, Department of Arctic and Marine Biology, UiT-The Arctic University of Norway, POBox 6050, Langnes, 9037, Tromsø, Norway., Marcin N; Arctic Infection Biology, Department of Arctic and Marine Biology, UiT-The Arctic University of Norway, POBox 6050, Langnes, 9037, Tromsø, Norway.; Clinique vétérinaire de l'abbatiale, 14 bis Rue Thibaut, 52220, Montier En Der, France., Nymo IH; Arctic Infection Biology, Department of Arctic and Marine Biology, UiT-The Arctic University of Norway, POBox 6050, Langnes, 9037, Tromsø, Norway., Josefsen TD; Norwegian Veterinary Institute, POBox 6050, Langnes, 9037, Tromsø, Norway.; Faculty of Bioscience and Aquaculture, Nord University, Bodø, Norway., Sørensen KK; Vascular Biology Research Group, Department of Medical Biology, Faculty of Health Sciences, UiT-The Arctic University of Norway, Tromsø, Norway., Mørk T; Norwegian Veterinary Institute, POBox 6050, Langnes, 9037, Tromsø, Norway.
Jazyk: angličtina
Zdroj: Acta veterinaria Scandinavica [Acta Vet Scand] 2017 Apr 24; Vol. 59 (1), pp. 23. Date of Electronic Publication: 2017 Apr 24.
DOI: 10.1186/s13028-017-0291-2
Abstrakt: Background: Infectious keratoconjunctivitis (IKC) is a transmissible disease in semi-domesticated Eurasian reindeer (Rangifer tarandus tarandus). It is regarded as multifactorial and a single causative pathogen has not yet been identified. From clinical outbreaks we have previously identified Cervid herpesvirus 2 (CvHV2) and Moraxella bovoculi as candidates for experimental investigations. Eighteen reindeer were inoculated in the right eye with CvHV2 (n = 5), M. bovoculi (n = 5), CvHV2 and M. bovoculi (n = 5) or sterile saline water (n = 3; controls).
Results: All animals inoculated with CvHv2, alone or in combination with M. bovoculi, showed raised body temperature, increased lacrimation, conjunctivitis, excretion of pus and periorbital oedema; clinical signs that increased in severity from day 2 post inoculation (p.i.) and throughout the experiment, until euthanasia 5-7 days p.i. Examination after euthanasia revealed corneal oedema, and three animals displayed a corneal ulcer. CvHV2 could be identified in swab samples from both the inoculated eye and the control eye from most animals and time points, indicating a viral spread from the inoculation site.
Conclusions: This study showed that CvHV2 alone and in combination with M. bovoculi was able to cause the characteristic clinical signs of IKC in reindeer, whereas inoculation of M. bovoculi alone, originally isolated from a reindeer with IKC, did not produce clinical signs. Previous studies have suggested that herding procedures, animal stress and subsequent reactivation of latent CvHV2 infection in older animals is a plausible mechanism for IKC outbreaks among reindeer calves and young animals in reindeer herds. However, further studies are needed to fully understand the infection biology and epidemiology associated with IKC in reindeer.
Databáze: MEDLINE
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