| Autor: |
Khodanovich MY; Research Institute of Biology and Biophysics, Tomsk State University, Tomsk, Russian Federation., Sorokina IV; Institute of Organic Chemistry, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russian Federation., Glazacheva VY; Research Institute of Biology and Biophysics, Tomsk State University, Tomsk, Russian Federation., Akulov AE; Research Institute of Biology and Biophysics, Tomsk State University, Tomsk, Russian Federation.; Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russian Federation., Nemirovich-Danchenko NM; Research Institute of Biology and Biophysics, Tomsk State University, Tomsk, Russian Federation., Romashchenko AV; Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russian Federation., Tolstikova TG; Institute of Organic Chemistry, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russian Federation., Mustafina LR; Siberian State Medical University, Tomsk, Russian Federation., Yarnykh VL; Research Institute of Biology and Biophysics, Tomsk State University, Tomsk, Russian Federation.; Department of Radiology, University of Washington, Seattle, WA, United States. |
| Abstrakt: |
Cuprizone-induced demyelination in mice is a frequently used model in preclinical multiple sclerosis research. A recent quantitative clinically-targeted MRI method, fast macromolecular proton fraction (MPF) mapping demonstrated a promise as a myelin biomarker in human and animal studies with a particular advantage of sensitivity to both white matter (WM) and gray matter (GM) demyelination. This study aimed to histologically validate the capability of MPF mapping to quantify myelin loss in brain tissues using the cuprizone demyelination model. Whole-brain MPF maps were obtained in vivo on an 11.7T animal MRI scanner from 7 cuprizone-treated and 7 control С57BL/6 mice using the fast single-point synthetic-reference method. Brain sections were histologically stained with Luxol Fast Blue (LFB) for myelin quantification. Significant (p < 0.05) demyelination in cuprizone-treated animals was found according to both LFB staining and MPF in all anatomical structures (corpus callosum, anterior commissure, internal capsule, thalamus, caudoputamen, and cortex). MPF strongly correlated with quantitative histology in all animals (r = 0.95, p < 0.001) as well as in treatment and control groups taken separately (r = 0.96, p = 0.002 and r = 0.93, p = 0.007, respectively). Close agreement between histological myelin staining and MPF suggests that fast MPF mapping enables robust and accurate quantitative assessment of demyelination in both WM and GM. |
