Modulation of the multidrug efflux pump EmrD-3 from Vibrio cholerae by Allium sativum extract and the bioactive agent allyl sulfide plus synergistic enhancement of antimicrobial susceptibility by A. sativum extract.

Autor: Bruns MM; Department of Biology, Eastern New Mexico University, Portales, NM, 88130, USA., Kakarla P; Department of Biology, Eastern New Mexico University, Portales, NM, 88130, USA., Floyd JT; Department of Biology, Eastern New Mexico University, Portales, NM, 88130, USA., Mukherjee MM; Department of Biology, Eastern New Mexico University, Portales, NM, 88130, USA., Ponce RC; Department of Biology, Eastern New Mexico University, Portales, NM, 88130, USA., Garcia JA; Department of Biology, Eastern New Mexico University, Portales, NM, 88130, USA., Ranaweera I; Department of Biology, Eastern New Mexico University, Portales, NM, 88130, USA., Sanford LM; Department of Biology, Eastern New Mexico University, Portales, NM, 88130, USA., Hernandez AJ; Department of Biology, Eastern New Mexico University, Portales, NM, 88130, USA., Willmon TM; Department of Biology, Eastern New Mexico University, Portales, NM, 88130, USA., Tolson GL; Department of Biology, Eastern New Mexico University, Portales, NM, 88130, USA., Varela MF; Department of Biology, Eastern New Mexico University, Portales, NM, 88130, USA. manuel.varela@enmu.edu.
Jazyk: angličtina
Zdroj: Archives of microbiology [Arch Microbiol] 2017 Oct; Vol. 199 (8), pp. 1103-1112. Date of Electronic Publication: 2017 Apr 21.
DOI: 10.1007/s00203-017-1378-x
Abstrakt: The causative agent of cholera, Vibrio cholerae, is a public health concern. Multidrug-resistant V. cholerae variants may reduce chemotherapeutic efficacies of severe cholera. We previously reported that the multidrug efflux pump EmrD-3 from V. cholerae confers resistance to multiple structurally distinct antimicrobials. Medicinal plant compounds are potential candidates for EmrD-3 efflux pump modulation. The antibacterial activities of garlic Allium sativum, although poorly understood, predicts that a main bioactive component, allyl sulfide, modulates EmrD-3 efflux. Thus, we tested whether A. sativum extract acts in synergy with antimicrobials and that a main bioactive component allyl sulfide inhibits EmrD-3 efflux. We found that A. sativum extract and allyl sulfide inhibited ethidium bromide efflux in cells harboring EmrD-3 and that A. sativum lowered the MICs of multiple antibacterials. We conclude that A. sativum and allyl sulfide inhibit EmrD-3 and that A. sativum extract synergistically enhances antibacterial agents.
Databáze: MEDLINE
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