Cacao Phylloplane: The First Battlefield against Moniliophthora perniciosa, Which Causes Witches' Broom Disease.

Autor:	Almeida DSM; First, third, fifth, and sixth authors: Proteomics Laboratory, Center of Biotechnology and Genetics, Department of Biological Sciences, State University of Santa Cruz, Rodovia Ilhéus-Itabuna km 16, 45652-900, Brazil; second author: Molecular Plant Pathology Laboratory, CEPLAC, Ilhéus, BA, Brazil; and fourth author: CIRAD-BIOS, UMR AGAP, Montpellier, France., Gramacho KP; First, third, fifth, and sixth authors: Proteomics Laboratory, Center of Biotechnology and Genetics, Department of Biological Sciences, State University of Santa Cruz, Rodovia Ilhéus-Itabuna km 16, 45652-900, Brazil; second author: Molecular Plant Pathology Laboratory, CEPLAC, Ilhéus, BA, Brazil; and fourth author: CIRAD-BIOS, UMR AGAP, Montpellier, France., Cardoso THS; First, third, fifth, and sixth authors: Proteomics Laboratory, Center of Biotechnology and Genetics, Department of Biological Sciences, State University of Santa Cruz, Rodovia Ilhéus-Itabuna km 16, 45652-900, Brazil; second author: Molecular Plant Pathology Laboratory, CEPLAC, Ilhéus, BA, Brazil; and fourth author: CIRAD-BIOS, UMR AGAP, Montpellier, France., Micheli F; First, third, fifth, and sixth authors: Proteomics Laboratory, Center of Biotechnology and Genetics, Department of Biological Sciences, State University of Santa Cruz, Rodovia Ilhéus-Itabuna km 16, 45652-900, Brazil; second author: Molecular Plant Pathology Laboratory, CEPLAC, Ilhéus, BA, Brazil; and fourth author: CIRAD-BIOS, UMR AGAP, Montpellier, France., Alvim FC; First, third, fifth, and sixth authors: Proteomics Laboratory, Center of Biotechnology and Genetics, Department of Biological Sciences, State University of Santa Cruz, Rodovia Ilhéus-Itabuna km 16, 45652-900, Brazil; second author: Molecular Plant Pathology Laboratory, CEPLAC, Ilhéus, BA, Brazil; and fourth author: CIRAD-BIOS, UMR AGAP, Montpellier, France., Pirovani CP; First, third, fifth, and sixth authors: Proteomics Laboratory, Center of Biotechnology and Genetics, Department of Biological Sciences, State University of Santa Cruz, Rodovia Ilhéus-Itabuna km 16, 45652-900, Brazil; second author: Molecular Plant Pathology Laboratory, CEPLAC, Ilhéus, BA, Brazil; and fourth author: CIRAD-BIOS, UMR AGAP, Montpellier, France.
Jazyk:	angličtina
Zdroj:	Phytopathology [Phytopathology] 2017 Jul; Vol. 107 (7), pp. 864-871. Date of Electronic Publication: 2017 May 31.
DOI:	10.1094/PHYTO-06-16-0226-R
Abstrakt:	The phylloplane is the first contact surface between Theobroma cacao and the fungus Moniliophthora perniciosa, which causes witches' broom disease (WBD). We evaluated the index of short glandular trichomes (SGT) in the cacao phylloplane and the effect of irrigation on the disease index of cacao genotypes with or without resistance to WBD, and identified proteins present in the phylloplane. The resistant genotype CCN51 and susceptible Catongo presented a mean index of 1,600 and 700 SGT cm -2 , respectively. The disease index in plants under drip irrigation was reduced by approximately 30% compared with plants under sprinkler irrigation prior to inoculation. Leaf water wash (LWW) of the cacao inhibited the germination of spores by up to 98%. Proteins from the LWW of CCN51 were analyzed by two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis followed by tandem mass spectrometry. The gel showed 71 spots and identified a total of 42 proteins (28 from the plant and 14 from bacteria). Proteins related to defense and synthesis of defense metabolites and involved in nucleic acid metabolism were identified. The results support the hypothesis that the proteins and water-soluble compounds secreted to the cacao phylloplane participate in the defense against pathogens. They also suggest that SGT can contribute to the resistance of cacao.
Databáze:	MEDLINE
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