Transplantation of inhibitory precursor cells from medial ganglionic eminence produces distinct responses in two different models of acute seizure induction.

Autor: Paiva DS; Laboratório de Neurofisiologia, Universidade Federal de São Paulo - UNIFESP, São Paulo, Brazil., Romariz SAA; Laboratório de Neurofisiologia, Universidade Federal de São Paulo - UNIFESP, São Paulo, Brazil., Valente MF; Laboratório de Neurofisiologia, Universidade Federal de São Paulo - UNIFESP, São Paulo, Brazil., Moraes LB; Laboratório de Neurofisiologia, Universidade Federal de São Paulo - UNIFESP, São Paulo, Brazil., Covolan L; Laboratório de Neurofisiologia, Universidade Federal de São Paulo - UNIFESP, São Paulo, Brazil., Calcagnotto ME; Universidade Federal do Rio Grande do Sul - UFRGS, Porto Alegre, Brazil., Monteiro Longo B; Laboratório de Neurofisiologia, Universidade Federal de São Paulo - UNIFESP, São Paulo, Brazil. Electronic address: biamonteironeuro@gmail.com.
Jazyk: angličtina
Zdroj: Epilepsy & behavior : E&B [Epilepsy Behav] 2017 May; Vol. 70 (Pt A), pp. 125-130. Date of Electronic Publication: 2017 Apr 17.
DOI: 10.1016/j.yebeh.2017.03.015
Abstrakt: Medial ganglionic eminence (MGE) is one of the sources of inhibitory interneurons during development. Following transplantation in postnatal developing brain, MGE cells can increase local inhibition suggesting a possible protection to GABAergic dysfunction in brain disorders, such as epilepsy. Since it has been shown that MGE-derived cells harvested as neurospheres are able to suppress seizures, it might be important to investigate whether these protective effects would change in different seizure models. Here, we used pentylenetetrazole-(PTZ) and maximal electroshock (MES)-induced seizure models to test whether the transplantation of MGE cells would increase the threshold to trigger acute seizures. When transplanted into the neocortex (layers 3-4) of neonatal mice (postnatal days 3-4), MGE cells were able to survive and were mainly found in piriform cortex, fimbria, and ventricular wall regions. Additionally, the number of GFP+ cells found in the brains of mice induced with PTZ and MES differed significantly and suggests proliferation and larger survival rate of MGE-transplanted cells after PTZ, but not MES-induced seizures. Following transplantation, there was a reduction in the number of animals presenting mild and severe seizures induced by PTZ. Furthermore, MGE-cell transplantation was able to increase threshold to seizures induced by PTZ, but was not able to prevent seizure spread induced by MES.
(Copyright © 2017 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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