Epstein-Barr virus ensures B cell survival by uniquely modulating apoptosis at early and late times after infection.

Autor:	Price AM; Department of Molecular Genetics and Microbiology, Center for Virology, Duke University School of Medicine, Durham, United States., Dai J; Department of Molecular Genetics and Microbiology, Center for Virology, Duke University School of Medicine, Durham, United States., Bazot Q; Molecular Virology, Division of Infectious Diseases, Department of Medicine, Imperial College London, London, United Kingdom., Patel L; Dana-Farber Cancer Institute, Harvard Medical School, Boston, United States., Nikitin PA; Department of Molecular Genetics and Microbiology, Center for Virology, Duke University School of Medicine, Durham, United States., Djavadian R; McArdle Laboratory for Cancer Research, University of Wisconsin School of Medicine and Public Health, Madison, United States.; Department of Medicine, University of Wisconsin School of Medicine and Public Health, Madison, United States., Winter PS; Department of Pharmacology and Cancer Biology, Duke University, Durham, United States.; Program in Genetics and Genomics, Duke University, Durham, United States., Salinas CA; Department of Molecular Genetics and Microbiology, Center for Virology, Duke University School of Medicine, Durham, United States., Barry AP; Department of Molecular Genetics and Microbiology, Center for Virology, Duke University School of Medicine, Durham, United States., Wood KC; Department of Pharmacology and Cancer Biology, Duke University, Durham, United States., Johannsen EC; McArdle Laboratory for Cancer Research, University of Wisconsin School of Medicine and Public Health, Madison, United States.; Department of Medicine, University of Wisconsin School of Medicine and Public Health, Madison, United States., Letai A; Dana-Farber Cancer Institute, Harvard Medical School, Boston, United States., Allday MJ; Molecular Virology, Division of Infectious Diseases, Department of Medicine, Imperial College London, London, United Kingdom., Luftig MA; Department of Molecular Genetics and Microbiology, Center for Virology, Duke University School of Medicine, Durham, United States.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2017 Apr 20; Vol. 6. Date of Electronic Publication: 2017 Apr 20.
DOI:	10.7554/eLife.22509
Abstrakt:	Latent Epstein-Barr virus (EBV) infection is causally linked to several human cancers. EBV expresses viral oncogenes that promote cell growth and inhibit the apoptotic response to uncontrolled proliferation. The EBV oncoprotein LMP1 constitutively activates NFκB and is critical for survival of EBV-immortalized B cells. However, during early infection EBV induces rapid B cell proliferation with low levels of LMP1 and little apoptosis. Therefore, we sought to define the mechanism of survival in the absence of LMP1/NFκB early after infection. We used BH3 profiling to query mitochondrial regulation of apoptosis and defined a transition from uninfected B cells (BCL-2) to early-infected (MCL-1/BCL-2) and immortalized cells (BFL-1). This dynamic change in B cell survival mechanisms is unique to virus-infected cells and relies on regulation of MCL-1 mitochondrial localization and BFL-1 transcription by the viral EBNA3A protein. This study defines a new role for EBNA3A in the suppression of apoptosis with implications for EBV lymphomagenesis.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu