Evaluation of two in-house immunoenzymatic tests to serodiagnose subclinical paratuberculisis in a sheep flock in Mexicali valley, Mexico.

Autor: Ponce Barraza E; a Instituto de Investigaciones en Ciencias Veterinarias , Universidad Autónoma de Baja California , Mexicali Baja California , Mexico., Cárdenas Reyna T; a Instituto de Investigaciones en Ciencias Veterinarias , Universidad Autónoma de Baja California , Mexicali Baja California , Mexico., Angulo C; b Immunology & Vaccinology Group , Centro de Investigaciones Biológicas en Noroeste , La Paz , México., Herrera Ramírez JC; a Instituto de Investigaciones en Ciencias Veterinarias , Universidad Autónoma de Baja California , Mexicali Baja California , Mexico., López GV; a Instituto de Investigaciones en Ciencias Veterinarias , Universidad Autónoma de Baja California , Mexicali Baja California , Mexico., Medina-Basulto GE; a Instituto de Investigaciones en Ciencias Veterinarias , Universidad Autónoma de Baja California , Mexicali Baja California , Mexico., Eda S; c Department of Forestry, Wildlife and Fisheries, Center for Wildlife Health , The University of Tennessee , Knoxville , Tennessee., Hori-Oshima S; a Instituto de Investigaciones en Ciencias Veterinarias , Universidad Autónoma de Baja California , Mexicali Baja California , Mexico.
Jazyk: angličtina
Zdroj: Journal of immunoassay & immunochemistry [J Immunoassay Immunochem] 2017; Vol. 38 (4), pp. 420-429. Date of Electronic Publication: 2017 Apr 19.
DOI: 10.1080/15321819.2017.1319862
Abstrakt: Paratuberculosis (PTB) or Johne's disease is a common ruminant infectious disease caused by Mycobacterium avium subsp. paratuberculosis (MAP). In this study, two MAP antigens were compared for their diagnostic utility to detect subclinical PTB in a sheep flock in Mexicali, Mexico. Sheep (n = 31) without clinical signs but positive on a direct fecal-polymerase chain reaction were tested with two preabsorbed in-house enzyme linked immunosorbent assays (ELISAs) using: (1) an ethanol-extracted surface lipid antigen (EVELISA) and (2) a protoplasmic antigen (ELISA-PPA). Sensitivities of the EVELISA and ELISA-PPA were 84% (95% CI; 66-95%) and 29% (95% CI; 14-48%), respectively. The EVELISA test could be a fast and effective way to identify subclinical ovine PTB for severely affected flocks.
Databáze: MEDLINE
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