| Autor: |
Sochivko DG; JSC Syntol, Moscow, 127550, Russia., Fedorov AA; Institute for Analytical Instrumentation, Russian Academy of Sciences, St. Petersburg, 198095, Russia. faa@bk.ru., Alekseev YI; All-Russia Research Institute of Agricultural Biotechnology, Moscow, 127550, Russia., Kurochkin VE; Institute for Analytical Instrumentation, Russian Academy of Sciences, St. Petersburg, 198095, Russia., Dubina MV; St. Petersburg National Research Academic University, St. Petersburg, 194021, Russia. |
| Abstrakt: |
The course of the real-time polymerase chain reaction (PCR) is determined by the temperature dependence of the kinetics of the component reactions, particularly the DNA strand hybridization. To investigate the effect of thermal processes on the reaction behavior, a mathematical model in which the variable rate constant of dissociation of "primer-single strand" complexes depends on temperature was proposed. The reaction medium temperature, which depends on time, was also introduced into the model. The proposed model of real-time PCR makes it possible to analyze different aspects of the reaction, which are important for the development of instruments and reagents for PCR. |
