| Autor: |
Hayden GE; Clinical Immunology Research Centre, University of Sydney, N.S.W., Australia., Walker KZ, Miller JF, Wotherspoon JS, Raison RL |
| Jazyk: |
angličtina |
| Zdroj: |
Cytometry [Cytometry] 1988 Jan; Vol. 9 (1), pp. 44-51. |
| DOI: |
10.1002/cyto.990090108 |
| Abstrakt: |
A method of two-colour immunofluorescence staining has been developed to allow the simultaneous analysis of both surface and cytoplasmic antigens. This involves the use of direct fluorochrome antibody conjugates for cell-surface antigen staining, followed by cell permeabilization and the staining of cytoplasmic antigens with biotinylated antibodies and streptavidin-fluorochrome conjugates. Fluorochrome-antibody conjugates bound to cell-surface epitopes were found not to be affected by the subsequent permeabilisation and cytoplasmic staining. This method was used to examine the surface phenotype of T cells expressing a cytoplasmic antigen, STA. STA is a unique determinant detected in activated human T cells by the monoclonal antibody K-1-21, which also recognizes a cross-reactive conformation-dependent epitope on human free kappa light chains. Cytometric analysis showed that STA is found in both Leu 2a+ cytotoxic/suppressor T cells and Leu 3a+ helper/inducer T cells but is not induced in the Leu 15+ population which contains suppressor T cells. STA was also shown to be an activation antigen in murine T cells. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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