Genetics and mapping of a new anthracnose resistance locus in Andean common bean Paloma.

Autor: de Lima Castro SA; Departamento de Agronomia, Universidade Estadual de Maringá, Maringá, Paraná, Brazil., Gonçalves-Vidigal MC; Departamento de Agronomia, Universidade Estadual de Maringá, Maringá, Paraná, Brazil. mcgvidigal@uem.br., Gilio TAS; Departamento de Agronomia, Universidade Estadual de Maringá, Maringá, Paraná, Brazil., Lacanallo GF; Departamento de Agronomia, Universidade Estadual de Maringá, Maringá, Paraná, Brazil., Valentini G; Departamento de Agronomia, Universidade Estadual de Maringá, Maringá, Paraná, Brazil., da Silva Ramos Martins V; Departamento de Agronomia, Universidade Estadual de Maringá, Maringá, Paraná, Brazil., Song Q; Soybean Genomics and Improvement Laboratory, United States Department of Agriculture, Agricultural Research Service, Beltsville, MD, USA., Galván MZ; CONICET, Laboratorio de Biotecnología, Estación Experimental Agropecuaria Salta, Instituto Nacional de Tecnología Agropecuaria, Cerrillos, Salta, Argentina., Hurtado-Gonzales OP; Soybean Genomics and Improvement Laboratory, United States Department of Agriculture, Agricultural Research Service, Beltsville, MD, USA., Pastor-Corrales MA; Soybean Genomics and Improvement Laboratory, United States Department of Agriculture, Agricultural Research Service, Beltsville, MD, USA. talo.pastor-corrales@ars.usda.gov.
Jazyk: angličtina
Zdroj: BMC genomics [BMC Genomics] 2017 Apr 18; Vol. 18 (1), pp. 306. Date of Electronic Publication: 2017 Apr 18.
DOI: 10.1186/s12864-017-3685-7
Abstrakt: Background: The Andean cultivar Paloma is resistant to Mesoamerican and Andean races of Colletotrichum lindemuthianum, the fungal pathogen that causes the destructive anthracnose disease in common bean. Remarkably, Paloma is resistant to Mesoamerican races 2047 and 3481, which are among the most virulent races of the anthracnose pathogen. Most genes conferring anthracnose resistance in common bean are overcome by these races. The genetic mapping and the relationship between the resistant Co-Pa gene of Paloma and previously characterized anthracnose resistance genes can be a great contribution for breeding programs.
Results: The inheritance of resistance studies for Paloma was performed in F 2 population from the cross Paloma (resistant) × Cornell 49-242 (susceptible) inoculated with race 2047, and in F 2 and F 2:3 generations from the cross Paloma (resistant) × PI 207262 (susceptible) inoculated with race 3481. The results of these studies demonstrated that a single dominant gene confers the resistance in Paloma. Allelism tests performed with multiple races of C. lindemuthianum showed that the resistance gene in Paloma, provisionally named Co-Pa, is independent from the anthracnose resistance genes Co-1, Co-2, Co-3, Co-4, Co-5, Co-6, Co-12, Co-13, Co-14, Co-15 and Co-16. Bulk segregant analysis using the SNP chip BARCBean6K_3 positioned the approximate location of Co-Pa in the lower arm of chromosome Pv01. Further mapping analysis located the Co-Pa gene at a 390 kb region of Pv01 flanked by SNP markers SS82 and SS83 at a distance of 1.3 and 2.1 cM, respectively.
Conclusions: The results presented here showed that Paloma cultivar has a new dominant gene conferring resistance to anthracnose, which is independent from those genes previously described. The linkage between the Co-Pa gene and the SS82 and SS83 SNP markers will be extremely important for marker-assisted introgression of the gene into elite cultivars in order to enhance resistance.
Databáze: MEDLINE
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