Regulation of Burkholderia cenocepacia biofilm formation by RpoN and the c-di-GMP effector BerB.

Autor: Fazli M; Department of Biology, Faculty of Science, University of Copenhagen, Copenhagen, Denmark.; Costerton Biofilm Center, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark., Rybtke M; Costerton Biofilm Center, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark., Steiner E; Department of Plant and Microbial Biology, University of Zurich, Zurich, Switzerland., Weidel E; Department of Drug Design and Optimization, Helmholtz Institute for Pharmaceutical Research Saarland, Saarbrücken, Germany., Berthelsen J; Costerton Biofilm Center, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark., Groizeleau J; Costerton Biofilm Center, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark., Bin W; School of Biological Sciences, Nanyang Technological University, Singapore, Singapore., Zhi BZ; School of Biological Sciences, Nanyang Technological University, Singapore, Singapore., Yaming Z; School of Biological Sciences, Nanyang Technological University, Singapore, Singapore., Kaever V; Research Core Unit Metabolomics, Institute of Pharmacology, Hannover Medical School, Hannover, Germany., Givskov M; Costerton Biofilm Center, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.; Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, Singapore., Hartmann RW; Department of Drug Design and Optimization, Helmholtz Institute for Pharmaceutical Research Saarland, Saarbrücken, Germany., Eberl L; Department of Plant and Microbial Biology, University of Zurich, Zurich, Switzerland., Tolker-Nielsen T; Costerton Biofilm Center, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Jazyk: angličtina
Zdroj: MicrobiologyOpen [Microbiologyopen] 2017 Aug; Vol. 6 (4). Date of Electronic Publication: 2017 Apr 16.
DOI: 10.1002/mbo3.480
Abstrakt: Knowledge about the molecular mechanisms that are involved in the regulation of biofilm formation is essential for the development of biofilm-control measures. It is well established that the nucleotide second messenger cyclic diguanosine monophosphate (c-di-GMP) is a positive regulator of biofilm formation in many bacteria, but more knowledge about c-di-GMP effectors is needed. We provide evidence that c-di-GMP, the alternative sigma factor RpoN (σ54), and the enhancer-binding protein BerB play a role in biofilm formation of Burkholderia cenocepacia by regulating the production of a biofilm-stabilizing exopolysaccharide. Our findings suggest that BerB binds c-di-GMP, and activates RpoN-dependent transcription of the berA gene coding for a c-di-GMP-responsive transcriptional regulator. An increased level of the BerA protein in turn induces the production of biofilm-stabilizing exopolysaccharide in response to high c-di-GMP levels. Our findings imply that the production of biofilm exopolysaccharide in B. cenocepacia is regulated through a cascade involving two consecutive transcription events that are both activated by c-di-GMP. This type of regulation may allow tight control of the expenditure of cellular resources.
(© 2017 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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