Scalable electrophysiology in intact small animals with nanoscale suspended electrode arrays.

Autor: Gonzales DL; Applied Physics Program, Rice University, 6100 Main Street, Houston, Texas 77005, USA.; Department of Electrical and Computer Engineering, Rice University, 6100 Main Street, Houston, Texas 77005, USA., Badhiwala KN; Department of Bioengineering, Rice University, 6100 Main Street, Houston, Texas 77005, USA., Vercosa DG; Applied Physics Program, Rice University, 6100 Main Street, Houston, Texas 77005, USA.; Department of Electrical and Computer Engineering, Rice University, 6100 Main Street, Houston, Texas 77005, USA., Avants BW; Department of Electrical and Computer Engineering, Rice University, 6100 Main Street, Houston, Texas 77005, USA., Liu Z; Department of BioSciences, Rice University, 6100 Main Street, Houston, Texas 77005, USA., Zhong W; Department of BioSciences, Rice University, 6100 Main Street, Houston, Texas 77005, USA., Robinson JT; Applied Physics Program, Rice University, 6100 Main Street, Houston, Texas 77005, USA.; Department of Electrical and Computer Engineering, Rice University, 6100 Main Street, Houston, Texas 77005, USA.; Department of Bioengineering, Rice University, 6100 Main Street, Houston, Texas 77005, USA.; Department of Neuroscience, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA.
Jazyk: angličtina
Zdroj: Nature nanotechnology [Nat Nanotechnol] 2017 Jul; Vol. 12 (7), pp. 684-691. Date of Electronic Publication: 2017 Apr 17.
DOI: 10.1038/nnano.2017.55
Abstrakt: Electrical measurements from large populations of animals would help reveal fundamental properties of the nervous system and neurological diseases. Small invertebrates are ideal for these large-scale studies; however, patch-clamp electrophysiology in microscopic animals typically requires invasive dissections and is low-throughput. To overcome these limitations, we present nano-SPEARs: suspended electrodes integrated into a scalable microfluidic device. Using this technology, we have made the first extracellular recordings of body-wall muscle electrophysiology inside an intact roundworm, Caenorhabditis elegans. We can also use nano-SPEARs to record from multiple animals in parallel and even from other species, such as Hydra littoralis. Furthermore, we use nano-SPEARs to establish the first electrophysiological phenotypes for C. elegans models for amyotrophic lateral sclerosis and Parkinson's disease, and show a partial rescue of the Parkinson's phenotype through drug treatment. These results demonstrate that nano-SPEARs provide the core technology for microchips that enable scalable, in vivo studies of neurobiology and neurological diseases.
Databáze: MEDLINE
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