Periapical Lesions Increase Macrophage Infiltration and Inflammatory Signaling in Muscle Tissue of Rats.

Autor:	Pereira RF; Programa de Pós-Graduação Multicêntrico em Ciências Fisiológicas-SBFis, Department of Basic Sciences, São Paulo State University (Unesp), School of Dentistry, Araçatuba, Brazil., Cintra LTA; Department of Restorative Dentistry, São Paulo State University (Unesp), School of Dentistry, Araçatuba, Brazil., Tessarin GWL; São Paulo State University (Unesp), Institute of Biosciences, Botucatu, Brazil., Chiba FY; Department of Child and Social Dentistry, São Paulo State University (Unesp), School of Dentistry, Araçatuba, Brazil. Electronic address: fernando_chiba@hotmail.com., de Lima Coutinho Mattera MS; Programa de Pós-Graduação Multicêntrico em Ciências Fisiológicas-SBFis, Department of Basic Sciences, São Paulo State University (Unesp), School of Dentistry, Araçatuba, Brazil., Scaramele NF; Department of Basic Sciences, São Paulo State University (Unesp), School of Dentistry, Araçatuba, Brazil., Tsosura TVS; Department of Basic Sciences, São Paulo State University (Unesp), School of Dentistry, Araçatuba, Brazil., Ervolino E; Department of Basic Sciences, São Paulo State University (Unesp), School of Dentistry, Araçatuba, Brazil., de Oliveira SHP; Programa de Pós-Graduação Multicêntrico em Ciências Fisiológicas-SBFis, Department of Basic Sciences, São Paulo State University (Unesp), School of Dentistry, Araçatuba, Brazil., Sumida DH; Programa de Pós-Graduação Multicêntrico em Ciências Fisiológicas-SBFis, Department of Basic Sciences, São Paulo State University (Unesp), School of Dentistry, Araçatuba, Brazil.
Jazyk:	angličtina
Zdroj:	Journal of endodontics [J Endod] 2017 Jun; Vol. 43 (6), pp. 982-988. Date of Electronic Publication: 2017 Apr 14.
DOI:	10.1016/j.joen.2017.01.030
Abstrakt:	Introduction: Our previous studies have shown that periapical lesions (PLs) in rats cause systemic disorders such as increased tumor necrosis factor-α plasma levels, insulin resistance, and impairment in insulin signal transduction in muscle tissue. However, the mechanisms involved in these alterations are not fully understood. Under chronic inflammatory conditions such as obesity, it has been shown that the skeletal muscle is affected by inflammation, and the number of resident macrophages that are associated with impairments of insulin action and sensitivity is increased. This study aimed to investigate the presence of macrophages, activation of inflammatory pathways in muscle tissue, glycemia, and insulinemia of rats with PLs. Methods: Sixty Wistar rats were distributed into a control group; a group with 1 PL (1PL), which was induced in the right maxillary first molar; and a group with 4 PLs (4PL), which were induced in the right upper and lower first and second molars. We quantified macrophage content by immunohistochemistry for the F4/80 protein. We evaluated Jun N-terminal kinase and IKKα/β phosphorylation status in the muscle tissue by Western blotting. Serum levels of lipopolysaccharide (LPS) and HSP70 and plasma levels of glucose and insulin were assessed by using commercial kits. Results: The 1PL and 4PL groups showed increase in macrophage content, IKKα/β, and Jun N-terminal kinase phosphorylation status, serum LPS and HSP70 levels, and insulin resistance and no changes in glycemia and insulinemia compared with the control group. There was no difference in these parameters between the 1PL and 4PL groups. Conclusions: PLs promoted an increase in macrophage infiltration, activation of inflammatory pathways in muscle tissue, and serum concentrations of HSP70 and LPS in rats. The present study improves the knowledge on the impact of oral inflammations on the development of systemic alteration, which can induce insulin resistance. (Copyright © 2017 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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