Novel keratin modified bacterial cellulose nanocomposite production and characterization for skin tissue engineering.

Autor: Keskin Z; Department of Biomedical Engineering, Faculty of Engineering, Izmir University of Economics, Izmir, Turkey., Sendemir Urkmez A; Department of Bioengineering, Faculty of Engineering, Ege University, Izmir, Turkey; Department of Biomedical Technologies, Ege University, Izmir, Turkey; Central Research Test and Analysis Laboratories Research and Application Center (EGEMATAL), Ege University, Izmir, Turkey., Hames EE; Department of Bioengineering, Faculty of Engineering, Ege University, Izmir, Turkey; Department of Biomedical Technologies, Ege University, Izmir, Turkey. Electronic address: esin.hames@ege.edu.tr.
Jazyk: angličtina
Zdroj: Materials science & engineering. C, Materials for biological applications [Mater Sci Eng C Mater Biol Appl] 2017 Jun 01; Vol. 75, pp. 1144-1153. Date of Electronic Publication: 2017 Mar 06.
DOI: 10.1016/j.msec.2017.03.035
Abstrakt: As it is known that bacterial cellulose (BC) is a biocompatible and natural biopolymer due to which it has a large set of biomedical applications. But still it lacks some desired properties, which limits its uses in many other applications. Therefore, the properties of BC need to be boosted up to an acceptable level. Here in this study for the first time, a new natural nanocomposite was produced by the incorporating keratin (isolated from human hair) to the BC (produced by Acetobacter xylinum) to enhance dermal fibroblast cells' attachment. Two different approaches were used in BC based nanocomposite production: in situ and post modifications. BC/keratin nanocomposites were characterized using SEM, FTIR, EDX, XRD, DSC and XPS analyses. Both production methods have yielded successful results for production of BC based nanocomposite-containing keratin. In vitro cell culture experiments performed with human skin keratinocytes and human skin fibroblast cells indicate the potential of the novel BC/keratin nanocomposites for use in skin tissue engineering.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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