[PCR analysis of the absolute number of copies of human chromosome 18 transcripts in liver and HepG2 cells].

Autor:	Kiseleva YY; Institute of Biomedical Chemistry, Moscow, Russia., Ptitsyn KG; Institute of Biomedical Chemistry, Moscow, Russia., Tikhonova OV; Institute of Biomedical Chemistry, Moscow, Russia., Radko SP; Institute of Biomedical Chemistry, Moscow, Russia., Kurbatov LK; Institute of Biomedical Chemistry, Moscow, Russia., Vakhrushev IV; Institute of Biomedical Chemistry, Moscow, Russia., Zgoda VG; Institute of Biomedical Chemistry, Moscow, Russia., Ponomarenko EA; Institute of Biomedical Chemistry, Moscow, Russia., Lisitsa AV; Institute of Biomedical Chemistry, Moscow, Russia., Archakov AI; Institute of Biomedical Chemistry, Moscow, Russia.
Jazyk:	ruština
Zdroj:	Biomeditsinskaia khimiia [Biomed Khim] 2017 Mar; Vol. 63 (2), pp. 147-153.
DOI:	10.18097/PBMC20176302147
Abstrakt:	Using reverse transcription in conjunction with the quantitative real-time PCR or digital droplet PCR, the transcriptome profiling of human chromosome 18 has been carried out in liver hepatocytes and hepatoblastoma cells (HepG2 cell line) in terms of the absolute number of each transcript per cell. The transcript abundance varies within the range of 0.006 to 9635 and 0.011 to 4819 copies per cell for HepG2 cell line and hepatocytes, respectively. The expression profiles for genes of chromosome 18 in hepatocytes and HepG2 cells were found to significantly correlate: the Spearman's correlation coefficient was equal to 0.81. The distribution of frequency of transcripts over their abundance was bimodal for HepG2 cells and unimodal for liver hepatocytes. Bioinformatic analysis of the differential gene expression has revealed that genes of chromosome 18, overexpressed in HepG2 cells compared to hepatocytes, are associated with cell division and cell adhesion processes. It is assumed that the enhanced expression of those genes in HepG2 cells is related to the proliferation activity of cultured cells. The differences in transcriptome profiles have to be taken into account when modelling liver hepatocytes with cultured HepG2 cells.
Databáze:	MEDLINE
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