Caffeoyl and coumaroyl derivatives from Acacia cochliacantha exhibit ovicidal activity against Haemonchus contortus.

Autor: Castillo-Mitre GF; Centro Universitario UAEM-Temascaltepec, Universidad Autónoma del Estado de México, km 67.5. Carr. Fed. Toluca-Tejupilco, CP 51300 Temascaltepec, Estado de México, Mexico., Olmedo-Juárez A; Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria (CENID PAVET-INIFAP), Carretera Federal Cuernavaca-Cuautla No. 8534/Col. Progreso, A.P. 206-CIVAC, C.P. 62550 Jiutepec, Morelos, Mexico. Electronic address: aolmedoj@gmail.com., Rojo-Rubio R; Centro Universitario UAEM-Temascaltepec, Universidad Autónoma del Estado de México, km 67.5. Carr. Fed. Toluca-Tejupilco, CP 51300 Temascaltepec, Estado de México, Mexico., González-Cortázar M; Centro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social, Argentina No. 1. Col. Centro, CP 62790 Xochitepec, Morelos, Mexico., Mendoza-de Gives P; Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria (CENID PAVET-INIFAP), Carretera Federal Cuernavaca-Cuautla No. 8534/Col. Progreso, A.P. 206-CIVAC, C.P. 62550 Jiutepec, Morelos, Mexico., Hernández-Beteta EE; Centro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social, Argentina No. 1. Col. Centro, CP 62790 Xochitepec, Morelos, Mexico., Reyes-Guerrero DE; Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria (CENID PAVET-INIFAP), Carretera Federal Cuernavaca-Cuautla No. 8534/Col. Progreso, A.P. 206-CIVAC, C.P. 62550 Jiutepec, Morelos, Mexico., López-Arellano ME; Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria (CENID PAVET-INIFAP), Carretera Federal Cuernavaca-Cuautla No. 8534/Col. Progreso, A.P. 206-CIVAC, C.P. 62550 Jiutepec, Morelos, Mexico., Vázquez-Armijo JF; Centro Universitario UAEM-Temascaltepec, Universidad Autónoma del Estado de México, km 67.5. Carr. Fed. Toluca-Tejupilco, CP 51300 Temascaltepec, Estado de México, Mexico., Ramírez-Vargas G; Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria (CENID PAVET-INIFAP), Carretera Federal Cuernavaca-Cuautla No. 8534/Col. Progreso, A.P. 206-CIVAC, C.P. 62550 Jiutepec, Morelos, Mexico., Zamilpa A; Centro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social, Argentina No. 1. Col. Centro, CP 62790 Xochitepec, Morelos, Mexico. Electronic address: azamilpa_2000@yahoo.com.mx.
Jazyk: angličtina
Zdroj: Journal of ethnopharmacology [J Ethnopharmacol] 2017 May 23; Vol. 204, pp. 125-131. Date of Electronic Publication: 2017 Apr 14.
DOI: 10.1016/j.jep.2017.04.010
Abstrakt: Ethnopharmacology Relevance: Acacia cochliacantha is a small tree whose foliage is traditionally used in Mexico for treatment of kidney pain, gastrointestinal illnesses and to kill intestinal parasites. In recent decades, the study of vegetal extracts has offered other possible alternatives for the control of Haemonchus contortus. Considering that this nematode affects dramatically the health and productivity of small ruminants, the aim of this study was to identify the anthelmintic compounds from A. cochliacantha hydro-alcoholic extract (HA-E) through an ovicidal test.
Material and Methods: In vitro egg hatch assay was conducted to determinate the anthelmintic effects of a HA-E (60g). Liquid-liquid ethyl acetate/water extraction gave two fractions (EtOAc-F, 1.92g; Aq-F; 58.1g). The less polar compounds from ethyl acetate fraction were extracted by addition of dichloromethane offering a precipitate phase (Mt-F, 1.25g) and a soluble mixture (DCMt-F 1.15g). All fractions were evaluated for ovicidal activity obtaining the egg hatching inhibition (EHI, 0.07-25mg/mL). Ivermectin (0.5mg/mL) was used as a reference drug (positive control), and distilled water, 2.5% DMSO and 2% methanol were used as negative controls. The isolated compounds from the most active fractions were subjected to spectroscopic ( 1 H NMR) Spectrometric (MS) and UV HPLC analysis in order to identify the bioactive compounds.
Results: The less polar treatments (AcOEt-F, DCMt-F, DCMt-P) showed the highest ovicidal activities (98-100% EHI; at 0.62-1.56mg/mL) and the major compounds found in these fractions were identified as caffeoyl and coumaroyl derivatives, including caffeic acid (1), p-coumaric acid (2), ferulic acid (3), methyl caffeate (4), methyl-p-coumarate (5), methyl ferulate (6) and quercetin. In case of the less active fractions (Aq-F, Mt-F) were constituted principally by glycosylated flavonoids.
Conclusion: These results show that caffeoyl and coumaroyl derivatives from Acacia cochliacantha leaves had promising anthelmintic activity against Haemonchus contortus. This leguminous may offer an alternative source for the control of gastrointestinal nematodes of small ruminants.
(Copyright © 2017 Elsevier Ireland Ltd. All rights reserved.)
Databáze: MEDLINE
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