A non-toxic dose of cobalt chloride blocks hair cells of the zebrafish lateral line.

Autor: Stewart WJ; Department of Biology, Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, FL, USA., Johansen JL; Department of Biology, Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, FL, USA., Liao JC; Department of Biology, Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, FL, USA. Electronic address: jliao@whitney.ufl.edu.
Jazyk: angličtina
Zdroj: Hearing research [Hear Res] 2017 Jul; Vol. 350, pp. 17-21. Date of Electronic Publication: 2017 Apr 12.
DOI: 10.1016/j.heares.2017.04.001
Abstrakt: Experiments on the flow-sensitive lateral line system of fishes have provided important insights into the function and sensory transduction of vertebrate hair cells. A common experimental approach has been to pharmacologically block lateral line hair cells and measure how behavior changes. Cobalt chloride (CoCl 2 ) blocks the lateral line by inhibiting calcium movement through the membrane channels of hair cells, but high concentrations can be toxic, making it unclear whether changes in behavior are due to a blocked lateral line or poor health. Here, we identify a non-toxic treatment of cobalt that completely blocks lateral line hair cells. We exposed 5-day post fertilization zebrafish larvae to CoCl 2 concentrations ranging from 1 to 20 mM for 15 min and measured 1) the spiking rate of the afferent neurons contacting hair cells and 2) the larvae's health and long-term survival. Our results show that a 15-min exposure to 5 mM CoCl 2 abolishes both spontaneous and evoked afferent firing. This treatment does not change swimming behavior, and results in >85% survival after 5 days. Weaker treatments of CoCl 2 did not eliminate afferent activity, while stronger treatments caused close to 50% mortality. Our work provides a guideline for future zebrafish investigations where physiological confirmation of a blocked lateral line system is required.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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