Development, validation and different approaches for the measurement uncertainty of a multi-class veterinary drugs residues LC-MS method for feeds.

Autor: Valese AC; Ministério da Agricultura, Pecuária e Abastecimento, Laboratório Nacional Agropecuário, Seção Laboratorial Avançada (SLAV/LANAGRO/RS), São José, SC 88102-600, Brazil; Universidade Federal de Santa Catarina (UFSC), Programa de Pós-graduação em Ciência de Alimentos, Centro de Ciências Agrárias, Florianópolis, SC 88034-000, Brazil., Molognoni L; Ministério da Agricultura, Pecuária e Abastecimento, Laboratório Nacional Agropecuário, Seção Laboratorial Avançada (SLAV/LANAGRO/RS), São José, SC 88102-600, Brazil; Instituto Catarinense de Sanidade Agropecuária (ICASA), Florianópolis, SC 88034-000, Brazil., de Souza NC; Ministério da Agricultura, Pecuária e Abastecimento, Laboratório Nacional Agropecuário, Seção Laboratorial Avançada (SLAV/LANAGRO/RS), São José, SC 88102-600, Brazil; UFSC, Departamento de Química, Campus Universitário Trindade, Florianópolis, SC 88404-900, Brazil., de Sá Ploêncio LA; Ministério da Agricultura, Pecuária e Abastecimento, Laboratório Nacional Agropecuário, Seção Laboratorial Avançada (SLAV/LANAGRO/RS), São José, SC 88102-600, Brazil; UFSC, Departamento de Química, Campus Universitário Trindade, Florianópolis, SC 88404-900, Brazil., Costa ACO; Universidade Federal de Santa Catarina (UFSC), Programa de Pós-graduação em Ciência de Alimentos, Centro de Ciências Agrárias, Florianópolis, SC 88034-000, Brazil., Barreto F; Ministério da Agricultura, Pecuária e Abastecimento, Laboratório Nacional Agropecuário, Seção Laboratorial Avançada (SLAV/LANAGRO/RS), São José, SC 88102-600, Brazil., Daguer H; Ministério da Agricultura, Pecuária e Abastecimento, Laboratório Nacional Agropecuário, Seção Laboratorial Avançada (SLAV/LANAGRO/RS), São José, SC 88102-600, Brazil. Electronic address: heitor.daguer@agricultura.gov.br.
Jazyk: angličtina
Zdroj: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] 2017 May 15; Vol. 1053, pp. 48-59. Date of Electronic Publication: 2017 Mar 25.
DOI: 10.1016/j.jchromb.2017.03.026
Abstrakt: A sensitive method for the simultaneous residues analysis of 62 veterinary drugs in feeds by liquid chromatography-tandem mass spectrometry has been developed and validated in accordance to Commission Decision 657/2002/EC. Additionally, limits of detection (LOD), limits of quantitation (LOQ), matrix effects and measurement uncertainty were also assessed. Extraction was performed for all analytes and respective internal standards in a single step and chromatographic separation was achieved in only 12min. LOQ were set to 0.63-5.00μgkg -1 (amphenicols), 0.63-30.00μgkg -1 (avermectins), 0.63μgkg -1 (benzimidazoles), 0.25-200.00μgkg -1 (coccidiostats), 0.63-200.00μgkg -1 (lincosamides and macrolides), 0.25-5.00μgkg -1 (nitrofurans), 0.63-20.00μgkg -1 (fluoroquinolones and quinolones), 15.00μgkg -1 (quinoxaline), 0.63-7.50μgkg -1 (sulfonamides), 0.63-20.00μgkg -1 (tetracyclines), 0.25μgkg -1 (β-agonists), and 30.00μgkg -1 (β-lactams). The top-down approach was adequate for the calculation of measurement uncertainty for all analytes, except the banned substances, which should be rather assessed by the bottom-up approach. Routine analysis of different types of feeds was then carried out. An interesting profile of residues of veterinary drugs among samples was revealed, enlightening the need for stricter control in producing animals. Among the total of 27 feed samples, 20 analytes could be detected/quantified, ranging from trace levels to very high concentrations. A high throughput screening/confirmatory method for the residue analysis of several veterinary drugs in feeds was proposed as a helpful control tool.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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