Searching for active mobile genetic elements in dsRNA fraction of Pinus sylvestris having witches broom abnormalities.

Autor:	Pochtovyy AA; Translational biomedicine laboratory, Gamaleya Research Center of Epidemiology and Microbiology, Russia.; Center for Molecular Biotechnology, Russian State Agrarian University-MTAA, Russia., Baranov OY; National Academy of Sciences of Belarus, Forest Research Institute, Gomel, Belarus., Rubel' IE; National Academy of Sciences of Belarus, Forest Research Institute, Gomel, Belarus., Razumova OA; National Academy of Sciences of Belarus, Forest Research Institute, Gomel, Belarus., Padutov VE; National Academy of Sciences of Belarus, Forest Research Institute, Gomel, Belarus., Khromov AV; Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Russia., Makhotenko AV; Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Russia., Tkachuk AP; Translational biomedicine laboratory, Gamaleya Research Center of Epidemiology and Microbiology, Russia., Makarov VV; A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Russia., Gushchin VA; Translational biomedicine laboratory, Gamaleya Research Center of Epidemiology and Microbiology, Russia.; A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Russia.
Jazyk:	angličtina
Zdroj:	Genomics data [Genom Data] 2017 Mar 28; Vol. 12, pp. 102-108. Date of Electronic Publication: 2017 Mar 28 (Print Publication: 2017).
DOI:	10.1016/j.gdata.2017.03.010
Abstrakt:	The most common type of coniferous mobile genetic elements are retrotransposons. Despite of their early positive impact on evolution of modern coniferous species they can have a significant negative impact for Forestry and breeding. Breaking genomic structural integrity mobile elements can cause phenotypic defects of plants. In this regard, the study of the diversity of coniferous mobile genetic elements is particularly interesting. In the present paper, we describe mobile genetic elements in dsRNA fraction of Pinus sylvestris having witches broom abnormalities. In result of assembled contigs analysis by RepeatMasker 70 mobile genetic elements were identified. A 68 of that were retroelements. Most of elements represented by Gypsy (16 contigs) and Copia (48 contigs). In 4 cases retroelements specific to Pinus taeda were identified. In most cases fragments of integrase (24), reverse transcriptase (22) and RNaseH (15) were identified. Results of the study may be of interest for coniferous breeding and genetic specialists. The raw data of these experiments have been deposited at NCBI under the accession number SAMN06185845.
Databáze:	MEDLINE
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