Molecular analysis of genetic diversity among vine accessions using DNA markers.

Autor: da Costa AF; Instituto Capixaba de Pesquisa, Assistência Técnica e Extensão Rural, Centro Regional de Desenvolvimento Rural, Centro Serrano, Domingos Martins, ES, Brasil., Teodoro PE; Laboratório de Biometria, Departamento de Biologia Geral, Universidade Federal de Viçosa, Viçosa, MG, Brasil eduteodoro@hotmail.com., Bhering LL; Laboratório de Biometria, Departamento de Biologia Geral, Universidade Federal de Viçosa, Viçosa, MG, Brasil., Tardin FD; Núcleo de Desenvolvimento de Cultivares, Embrapa Milho e Sorgo, Sinop, MT, Brasil., Daher RF; Laboratório de Melhoramento Genético Vegetal, , , Brasil., Campos WF; Laboratório de Melhoramento Genético Vegetal, , , Brasil., Viana AP; Laboratório de Melhoramento Genético Vegetal, , , Brasil., Pereira MG; Laboratório de Melhoramento Genético Vegetal, , , Brasil.
Jazyk: angličtina
Zdroj: Genetics and molecular research : GMR [Genet Mol Res] 2017 Apr 13; Vol. 16 (2). Date of Electronic Publication: 2017 Apr 13.
DOI: 10.4238/gmr16029586
Abstrakt: Viticulture presents a number of economic and social advantages, such as increasing employment levels and fixing the labor force in rural areas. With the aim of initiating a program of genetic improvement in grapevine from the State University of the state of Rio de Janeiro North Darcy Ribeiro, genetic diversity between 40 genotypes (varieties, rootstock, and species of different subgenera) was evaluated using Random amplified polymorphic DNA (RAPD) molecular markers. We built a matrix of binary data, whereby the presence of a band was assigned as "1" and the absence of a band was assigned as "0." The genetic distance was calculated between pairs of genotypes based on the arithmetic complement from the Jaccard Index. The results revealed the presence of considerable variability in the collection. Analysis of the genetic dissimilarity matrix revealed that the most dissimilar genotypes were Rupestris du Lot and Vitis rotundifolia because they were the most genetically distant (0.5972). The most similar were genotypes 31 (unidentified) and Rupestris du lot, which showed zero distance, confirming the results of field observations. A duplicate was confirmed, consistent with field observations, and a short distance was found between the variety 'Italy' and its mutation, 'Ruby'. The grouping methods used were somewhat concordant.
Databáze: MEDLINE