Comparison of ePlex Respiratory Pathogen Panel with Laboratory-Developed Real-Time PCR Assays for Detection of Respiratory Pathogens.

Autor: Nijhuis RHT; Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands r.h.t.nijhuis@lumc.nl., Guerendiain D; Specialist Virology Center, Royal Infirmary of Edinburgh, Edinburgh, United Kingdom., Claas ECJ; Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands., Templeton KE; Specialist Virology Center, Royal Infirmary of Edinburgh, Edinburgh, United Kingdom.
Jazyk: angličtina
Zdroj: Journal of clinical microbiology [J Clin Microbiol] 2017 Jun; Vol. 55 (6), pp. 1938-1945. Date of Electronic Publication: 2017 Apr 12.
DOI: 10.1128/JCM.00221-17
Abstrakt: Infections of the respiratory tract can be caused by a diversity of pathogens, both viral and bacterial. Rapid microbiological diagnosis ensures appropriate antimicrobial therapy as well as effective implementation of isolation precautions. The ePlex respiratory pathogen panel (RP panel) is a novel molecular biology-based assay, developed by GenMark Diagnostics, Inc. (Carlsbad, CA), to be performed within a single cartridge for the diagnosis of 25 respiratory pathogens (viral and bacterial). The objective of this study was to compare the performance of the RP panel with those of laboratory-developed real-time PCR assays, using a variety of previously collected clinical respiratory specimens. A total of 343 clinical specimens as well as 29 external quality assessment (EQA) specimens and 2 different Middle East respiratory syndrome coronavirus isolates have been assessed in this study. The RP panel showed an agreement of 97.4% with the real-time PCR assay regarding 464 pathogens found in the clinical specimens. All pathogens present in clinical samples and EQA samples with a threshold cycle ( C T ) value of <30 were detected correctly using the RP panel. The RP panel detected 17 additional pathogens, 7 of which could be confirmed by discrepant testing. In conclusion, this study shows excellent performance of the RP panel in comparison to real-time PCR assays for the detection of respiratory pathogens. The ePlex system provided a large amount of useful diagnostic data within a short time frame, with minimal hands-on time, and can therefore potentially be used for rapid diagnostic sample-to-answer testing, in either a laboratory or a decentralized setting.
(Copyright © 2017 Nijhuis et al.)
Databáze: MEDLINE
Externí odkaz: