Regulatory functions and chromatin loading dynamics of linker histone H1 during endoreplication in Drosophila .

Autor:	Andreyeva EN; Institute of Molecular and Cellular Biology, Siberian Branch of the Russian Academy of Sciences, Novosibirsk 630090, Russian Federation., Bernardo TJ; Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA., Kolesnikova TD; Institute of Molecular and Cellular Biology, Siberian Branch of the Russian Academy of Sciences, Novosibirsk 630090, Russian Federation.; Novosibirsk State University, Novosibirsk 630090, Russian Federation., Lu X; Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA., Yarinich LA; Institute of Molecular and Cellular Biology, Siberian Branch of the Russian Academy of Sciences, Novosibirsk 630090, Russian Federation.; Novosibirsk State University, Novosibirsk 630090, Russian Federation., Bartholdy BA; Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA., Guo X; Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA., Posukh OV; Institute of Molecular and Cellular Biology, Siberian Branch of the Russian Academy of Sciences, Novosibirsk 630090, Russian Federation., Healton S; Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA., Willcockson MA; Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA., Pindyurin AV; Institute of Molecular and Cellular Biology, Siberian Branch of the Russian Academy of Sciences, Novosibirsk 630090, Russian Federation., Zhimulev IF; Institute of Molecular and Cellular Biology, Siberian Branch of the Russian Academy of Sciences, Novosibirsk 630090, Russian Federation.; Novosibirsk State University, Novosibirsk 630090, Russian Federation., Skoultchi AI; Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA., Fyodorov DV; Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Jazyk:	angličtina
Zdroj:	Genes & development [Genes Dev] 2017 Mar 15; Vol. 31 (6), pp. 603-616.
DOI:	10.1101/gad.295717.116
Abstrakt:	Eukaryotic DNA replicates asynchronously, with discrete genomic loci replicating during different stages of S phase. Drosophila larval tissues undergo endoreplication without cell division, and the latest replicating regions occasionally fail to complete endoreplication, resulting in underreplicated domains of polytene chromosomes. Here we show that linker histone H1 is required for the underreplication (UR) phenomenon in Drosophila salivary glands. H1 directly interacts with the Suppressor of UR (SUUR) protein and is required for SUUR binding to chromatin in vivo. These observations implicate H1 as a critical factor in the formation of underreplicated regions and an upstream effector of SUUR. We also demonstrate that the localization of H1 in chromatin changes profoundly during the endocycle. At the onset of endocycle S (endo-S) phase, H1 is heavily and specifically loaded into late replicating genomic regions and is then redistributed during the course of endoreplication. Our data suggest that cell cycle-dependent chromosome occupancy of H1 is governed by several independent processes. In addition to the ubiquitous replication-related disassembly and reassembly of chromatin, H1 is deposited into chromatin through a novel pathway that is replication-independent, rapid, and locus-specific. This cell cycle-directed dynamic localization of H1 in chromatin may play an important role in the regulation of DNA replication timing. (© 2017 Andreyeva et al.; Published by Cold Spring Harbor Laboratory Press.)
Databáze:	MEDLINE
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