Direct effects of interleukin-7 on the function of human T cells in vitro.

Autor: Shmarov VA; Immanuel Kant Baltic Federal University, Kaliningrad, Russia., Malashchenko VV; Immanuel Kant Baltic Federal University, Kaliningrad, Russia., Meniailo ME; Immanuel Kant Baltic Federal University, Kaliningrad, Russia., Gazatova ND; Immanuel Kant Baltic Federal University, Kaliningrad, Russia., Todosenko NM; Immanuel Kant Baltic Federal University, Kaliningrad, Russia., Melashchenko OB; Immanuel Kant Baltic Federal University, Kaliningrad, Russia., Goncharov AG; Immanuel Kant Baltic Federal University, Kaliningrad, Russia., Seledtsov VI; Immanuel Kant Baltic Federal University, Kaliningrad, Russia.
Jazyk: angličtina
Zdroj: European cytokine network [Eur Cytokine Netw] 2016 Nov 01; Vol. 27 (4), pp. 102-107.
DOI: 10.1684/ecn.2016.0385
Abstrakt: СD3+ T lymphocytes were isolated by positive magnetic separation from the peripheral blood of healthy donors. In the absence of any additional activating stimuli, interleukin-7 (IL-7) was shown to augment the levels of T cells expressing CD25 activation marker both in СD4-positive and in CD4-negative effector memory (CD45RA - CD197 - ) T cell subsets, as well as in terminally differentiated (CD45RA + CD197 - ) Т cells, without significantly affecting the activation status of naive (CD45RA + CD197 + ) and central memory (CD45RA - CD197 + ) T cells. In addition, IL-7 noticeably enhanced the production of IL-2, interferon-γ (IFN-γ), and IL-10, but not IL-4, in T cells. The direct effects of IL-7 on T cell activation induced in vitro by MACSiBead™ particles coated with CD2, CD3, and CD28 antibodies (Abs) were also investigated. Upon cell activation, IL-7 significantly augmented the levels of CD25+ T cells in naive (CD45RA+CD197+), central memory (CD45RA - CD197 + ), and effector memory (CD45RA - CD197 - ) T-cell compartments. In addition, IL-7 facilitated activation of СD4 - (but not CD4 + ) terminally differentiated effector (CD45RA + CD197 - ) Т cells. Finally, IL-7 was found to upregulate the production of IL-2, IFN-γ, IL-4, and IL-10 by activated T cells. In conclusion, we speculate that IL-7 is capable of enhancing functional T cell activity without causing significant functional inbalance between various T cell subsets.
Databáze: MEDLINE
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