Cartilage Tissue Engineering by the 3D Bioprinting of iPS Cells in a Nanocellulose/Alginate Bioink.

Autor: Nguyen D; Chemical Biology, Dept. of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden.; Institute of Biomedicine at Sahlgrenska Academy, Department of Clinical Chemistry and Transfusion Medicine, University of Gothenburg, Gothenburg, Sweden., Hägg DA; 3D Bioprinting Center, Dept. of Chemistry and Chemical Engineering, Chalmers University of Technology, Gothenburg, Sweden., Forsman A; Institute of Biomedicine at Sahlgrenska Academy, Department of Clinical Chemistry and Transfusion Medicine, University of Gothenburg, Gothenburg, Sweden., Ekholm J; Institute of Biomedicine at Sahlgrenska Academy, Department of Clinical Chemistry and Transfusion Medicine, University of Gothenburg, Gothenburg, Sweden., Nimkingratana P; Institute of Biomedicine at Sahlgrenska Academy, Department of Clinical Chemistry and Transfusion Medicine, University of Gothenburg, Gothenburg, Sweden., Brantsing C; Institute of Biomedicine at Sahlgrenska Academy, Department of Clinical Chemistry and Transfusion Medicine, University of Gothenburg, Gothenburg, Sweden., Kalogeropoulos T; 3D Bioprinting Center, Dept. of Chemistry and Chemical Engineering, Chalmers University of Technology, Gothenburg, Sweden., Zaunz S; 3D Bioprinting Center, Dept. of Chemistry and Chemical Engineering, Chalmers University of Technology, Gothenburg, Sweden., Concaro S; Cartilage Repair Unit, University of Gothenburg, Region Halland Orthopaedics, Kungsbacka Hospital, Kungsbacka, Sweden., Brittberg M; Cartilage Repair Unit, University of Gothenburg, Region Halland Orthopaedics, Kungsbacka Hospital, Kungsbacka, Sweden., Lindahl A; Institute of Biomedicine at Sahlgrenska Academy, Department of Clinical Chemistry and Transfusion Medicine, University of Gothenburg, Gothenburg, Sweden., Gatenholm P; 3D Bioprinting Center, Dept. of Chemistry and Chemical Engineering, Chalmers University of Technology, Gothenburg, Sweden.; Wallenberg Wood Science Center, Chalmers University of Technology, Gothenburg, Sweden., Enejder A; Chemical Biology, Dept. of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden., Simonsson S; Institute of Biomedicine at Sahlgrenska Academy, Department of Clinical Chemistry and Transfusion Medicine, University of Gothenburg, Gothenburg, Sweden. stina.simonsson@gu.se.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2017 Apr 06; Vol. 7 (1), pp. 658. Date of Electronic Publication: 2017 Apr 06.
DOI: 10.1038/s41598-017-00690-y
Abstrakt: Cartilage lesions can progress into secondary osteoarthritis and cause severe clinical problems in numerous patients. As a prospective treatment of such lesions, human-derived induced pluripotent stem cells (iPSCs) were shown to be 3D bioprinted into cartilage mimics using a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes. Two bioinks were investigated: NFC with alginate (NFC/A) or hyaluronic acid (NFC/HA). Low proliferation and phenotypic changes away from pluripotency were seen in the case of NFC/HA. However, in the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression was observed in 3D -bioprinted NFC/A (60/40, dry weight % relation) constructs. Moreover, a marked increase in cell number within the cartilaginous tissue was detected by 2-photon fluorescence microscopy, indicating the importance of high cell densities in the pursuit of achieving good survival after printing. We conclude that NFC/A bioink is suitable for bioprinting iPSCs to support cartilage production in co-cultures with irradiated chondrocytes.
Databáze: MEDLINE