| Autor: |
Platts-Mills JA; Division of Infectious Diseases and International Health, University of Virginia, Charlottesville, VA; and jp5t@virginia.edu., Taniuchi M; Division of Infectious Diseases and International Health, University of Virginia, Charlottesville, VA; and., Uddin MJ; International Center for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh., Sobuz SU; International Center for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh., Mahfuz M; International Center for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh., Gaffar SA; International Center for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh., Mondal D; International Center for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh., Hossain MI; International Center for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh., Islam MM; International Center for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh., Ahmed AS; International Center for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh., Petri WA; Division of Infectious Diseases and International Health, University of Virginia, Charlottesville, VA; and., Haque R; International Center for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh., Houpt ER; Division of Infectious Diseases and International Health, University of Virginia, Charlottesville, VA; and., Ahmed T; International Center for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh. |
| Abstrakt: |
Background: Early exposure to enteropathogens has been associated with malnutrition in children in low-resource settings. However, the contribution of individual enteropathogens remains poorly defined. Molecular diagnostics offer an increase in sensitivity for detecting enteropathogens but have not been comprehensively applied to studies of malnutrition. Objective: We sought to identify enteropathogens associated with malnutrition in Bangladesh. Design: Malnourished children [weight-for-age z score (WAZ) <-2] aged 6-23 mo in Dhaka, Bangladesh, and identified by active community surveillance were enrolled as cases, and normal-weight children (WAZ >-1) of the same age and from the same community were enrolled as controls. Stools were collected at enrollment and, for cases, after a 5-mo nutritional intervention. Enrollment and follow-up stools were tested by quantitative polymerase chain reaction for 32 enteropathogens with the use of a custom-developed TaqMan Array Card. Results: Enteropathogen testing was performed on 486 cases and 442 controls upon enrollment and 365 cases at follow-up. At enrollment, the detection of enteroaggregative Escherichia coli (OR: 1.39; 95% CI: 1.05, 1.83), Campylobacter spp. (OR: 1.46; 95% CI: 1.11, 1.91), heat-labile enterotoxin-producing E. coli (OR: 1.55; 95% CI: 1.04, 2.33), Shigella /enteroinvasive E. coli (OR: 1.65; 95% CI: 1.10, 2.46), norovirus genogroup I (OR: 1.66; 95% CI: 1.23, 2.25), and Giardia (OR: 1.73; 95% CI: 1.20, 2.49) were associated with malnourished cases, and the total burden of these pathogens remained associated with malnutrition after adjusting for sociodemographic factors. The number of these pathogens at follow-up was negatively associated with the change in WAZ during the intervention (-0.10 change in WAZ per pathogen detected; 95% CI: -0.14, -0.06), whereas the number at enrollment was positively associated with the change in WAZ (0.05 change in WAZ per pathogen detected; 95% CI: 0.00, 0.10). Conclusions: A subset of enteropathogens was associated with malnutrition in this setting. Broad interventions designed to reduce the burden of infection with these pathogens are needed. This trial was registered at clinicaltrials.gov as NCT02441426. |
