Molecular displaying of differential immunoresponse to various infections of amniotic epithelia.

Autor: Nemr W; Department of Radiation Microbiology, National Centre for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt., Bashandy M; Department of Radiation Microbiology, National Centre for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt., Araby E; Department of Radiation Microbiology, National Centre for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt., Khamiss O; Department of Animal Biotechnology, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City (USC), Sadat, Egypt.
Jazyk: angličtina
Zdroj: American journal of reproductive immunology (New York, N.Y. : 1989) [Am J Reprod Immunol] 2017 Jun; Vol. 77 (6). Date of Electronic Publication: 2017 Apr 05.
DOI: 10.1111/aji.12662
Abstrakt: Problem: Molecular displaying for the interaction of innate immune cells against pathogen is important for knowing their defense mechanism. This study aimed to visualize the differential gene expression profiles of amniotic epithelium, in response to various infections, using simple reverse transcription polymerase chain reaction (RT-PCR) techniques.
Method of Study: Antimicrobial activity of human amniotic epithelial cells (HAECs) was verified against three different models of pathogenic microorganisms. RNA was extracted from infected and non-infected cells. Transcripts were visualized by two methods of RT-PCR; one of them targets β-defensin 2 (BDEF2). The other is a novel design method of RNA fingerprinting based on differential length amplification of transcripts (DifLAT) to polymorphism more than 69 coding sequences for antimicrobial proteins.
Results: The semiquantitative gel analysis indicated that BDEF2 was upregulated during all infections. DifLAT experiment visualized different patterns of HAECs transcripts for each case of infection.
Conclusion: This study proved that HAECs uses alternative gene expression profiles for fighting different pathogens.
(© 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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