RNA-Seq for Gene Expression Profiling of Human Necrotizing Enterocolitis: a Pilot Study.

Autor:	Jung K; Department of Surgery, Jeju National University Hospital, Jeju, Korea., Koh I; Department of Physiology, College of Medicine, Hanyang University, Seoul, Korea., Kim JH; Research Institute for Basic Science, Sogang University, Seoul, Korea., Cheong HS; Department of Genetic Epidemiology, SNP Genetics, Inc., Seoul, Korea., Park T; Department of Surgery, Gyeongsang National University Hospital, Jinju, Korea., Nam SH; Department of Surgery, Donga University Hospital, Busan, Korea., Jung SM; Department of Surgery, Konkuk University Medical Center, Konkuk University School of Medicine, Seoul, Korea., Sio CA; Department of Surgery, Seoul National University Bundang Hospital, Seongnam, Korea., Kim SY; Department of Surgery, Donga University Hospital, Busan, Korea., Jung E; Department of Neonatology, Seoul National University Bundang Hospital, Seongnam, Korea., Lee B; Department of Neonatology, Seoul National University Bundang Hospital, Seongnam, Korea., Kim HR; Department of Neonatology, Seoul National University Bundang Hospital, Seongnam, Korea., Shin E; Department of Pathology, Seoul National University Bundang Hospital, Seongnam, Korea., Jung SE; Department of Pediatric Surgery, Seoul National University Children's Hospital, Seoul, Korea., Choi CW; Department of Neonatology, Seoul National University Bundang Hospital, Seongnam, Korea., Kim BI; Department of Neonatology, Seoul National University Bundang Hospital, Seongnam, Korea., Jung E; Department of Surgery, Gyemyoung University Dongsan Hospital, Daegu, Korea., Shin HD; Research Institute for Basic Science, Sogang University, Seoul, Korea.; Department of Life Science, Sogang University, Seoul, Korea. hdshin@sogang.ac.kr.
Jazyk:	angličtina
Zdroj:	Journal of Korean medical science [J Korean Med Sci] 2017 May; Vol. 32 (5), pp. 817-824.
DOI:	10.3346/jkms.2017.32.5.817
Abstrakt:	Necrotizing enterocolitis (NEC) characterized by inflammatory intestinal necrosis is a major cause of mortality and morbidity in newborns. Deep RNA sequencing (RNA-Seq) has recently emerged as a powerful technology enabling better quantification of gene expression than microarrays with a lower background signal. A total of 10 transcriptomes from 5 pairs of NEC lesions and adjacent normal tissues obtained from preterm infants with NEC were analyzed. As a result, a total of 65 genes (57 down-regulated and 8 up-regulated) revealed significantly different expression levels in the NEC lesion compared to the adjacent normal region, based on a significance at fold change ≥ 1.5 and P ≤ 0.05. The most significant gene, DPF3 (P < 0.001), has recently been reported to have differential expressions in colon segments. Our gene ontology analysis between NEC lesion and adjacent normal tissues showed that down-regulated genes were included in nervous system development with the most significance (P = 9.3 × 10⁻⁷; P(corr) = 0.0003). In further pathway analysis using Pathway Express based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) database, genes involved in thyroid cancer and axon guidance were predicted to be associated with different expression (P(corr) = 0.008 and 0.020, respectively). Although further replications using a larger sample size and functional evaluations are needed, our results suggest that altered gene expression and the genes' involved functional pathways and categories may provide insight into NEC development and aid in future research. Competing Interests: The authors have no potential conflicts of interest to disclose. (© 2017 The Korean Academy of Medical Sciences.)
Databáze:	MEDLINE
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