Identification of autoreactive B cells with labeled nucleosomes.

Autor: Gies V; CNRS UPR 3572 'Immunopathology and Therapeutic Chemistry'/Laboratory of Excellence Medalis, Institute of Molecular and Cellular Biology (IBMC), Strasbourg, France.; Department of Clinical Immunology and Internal Medicine, National Reference Center for Autoimmune Diseases, Hôpitaux Universitaires de Strasbourg, Strasbourg, France., Wagner A; Laboratory of Functional ChemoSystems, CNRS-University of Strasbourg UMR 7199/Laboratory of Excellence MEDALIS, Faculté de Pharmacie, Université de Strasbourg, 74 route du Rhin, 67400, Illkirch, France., Seifert C; CNRS UPR 3572 'Immunopathology and Therapeutic Chemistry'/Laboratory of Excellence Medalis, Institute of Molecular and Cellular Biology (IBMC), Strasbourg, France., Guffroy A; CNRS UPR 3572 'Immunopathology and Therapeutic Chemistry'/Laboratory of Excellence Medalis, Institute of Molecular and Cellular Biology (IBMC), Strasbourg, France.; Department of Clinical Immunology and Internal Medicine, National Reference Center for Autoimmune Diseases, Hôpitaux Universitaires de Strasbourg, Strasbourg, France., Fauny JD; CNRS UPR 3572 'Immunopathology and Therapeutic Chemistry'/Laboratory of Excellence Medalis, Institute of Molecular and Cellular Biology (IBMC), Strasbourg, France., Knapp AM; CNRS UPR 3572 'Immunopathology and Therapeutic Chemistry'/Laboratory of Excellence Medalis, Institute of Molecular and Cellular Biology (IBMC), Strasbourg, France., Pasquali JL; CNRS UPR 3572 'Immunopathology and Therapeutic Chemistry'/Laboratory of Excellence Medalis, Institute of Molecular and Cellular Biology (IBMC), Strasbourg, France.; Department of Clinical Immunology and Internal Medicine, National Reference Center for Autoimmune Diseases, Hôpitaux Universitaires de Strasbourg, Strasbourg, France.; UFR Médecine, Université de Strasbourg, Strasbourg, France., Martin T; CNRS UPR 3572 'Immunopathology and Therapeutic Chemistry'/Laboratory of Excellence Medalis, Institute of Molecular and Cellular Biology (IBMC), Strasbourg, France.; Department of Clinical Immunology and Internal Medicine, National Reference Center for Autoimmune Diseases, Hôpitaux Universitaires de Strasbourg, Strasbourg, France.; UFR Médecine, Université de Strasbourg, Strasbourg, France., Dumortier H; CNRS UPR 3572 'Immunopathology and Therapeutic Chemistry'/Laboratory of Excellence Medalis, Institute of Molecular and Cellular Biology (IBMC), Strasbourg, France., Korganow AS; CNRS UPR 3572 'Immunopathology and Therapeutic Chemistry'/Laboratory of Excellence Medalis, Institute of Molecular and Cellular Biology (IBMC), Strasbourg, France.; Department of Clinical Immunology and Internal Medicine, National Reference Center for Autoimmune Diseases, Hôpitaux Universitaires de Strasbourg, Strasbourg, France.; UFR Médecine, Université de Strasbourg, Strasbourg, France., Soulas-Sprauel P; CNRS UPR 3572 'Immunopathology and Therapeutic Chemistry'/Laboratory of Excellence Medalis, Institute of Molecular and Cellular Biology (IBMC), Strasbourg, France. pauline.soulas@ibmc-cnrs.unistra.fr.; Department of Clinical Immunology and Internal Medicine, National Reference Center for Autoimmune Diseases, Hôpitaux Universitaires de Strasbourg, Strasbourg, France. pauline.soulas@ibmc-cnrs.unistra.fr.; UFR Sciences pharmaceutiques, Université de Strasbourg, Illkirch-Graffenstaden, France. pauline.soulas@ibmc-cnrs.unistra.fr.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2017 Apr 04; Vol. 7 (1), pp. 602. Date of Electronic Publication: 2017 Apr 04.
DOI: 10.1038/s41598-017-00664-0
Abstrakt: The pathogenesis of autoimmune diseases has not been completely elucidated yet, and only a few specific treatments have been developed so far. In autoimmune diseases mediated by pathogenic autoantibodies, such as systemic lupus erythematosus, the specific detection and analysis of autoreactive B cells is crucial for a better understanding of the physiopathology. Biological characterization of these cells may help to define new therapeutic targets. Very few techniques allowing the precise detection of autoreactive B cells have been described so far. Herein we propose a new flow cytometry technique for specific detection of anti-nucleosome B cells, which secrete autoantibodies in systemic lupus erythematosus, using labeled nucleosomes. We produced different fluorochrome-labeled nucleosomes, characterized them, and finally tested them in flow cytometry. Nucleosomes labeled via the cysteines present in H3 histone specifically bind to autoreactive B cells in the anti-DNA transgenic B6.56R mice model. The present work validates the use of fluorochrome-labeled nucleosomes via cysteines to identify anti-nucleosome B cells and offers new opportunities for the description of autoreactive B cell phenotype.
Databáze: MEDLINE
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