Residual determination of anesthetic menthol in fishes by SDME/GC-MS.

Autor: Botrel BM; Department of Chemistry, Federal University of Lavras, 37200-000 Lavras, MG, Brazil. Electronic address: biamesquitacoelho@yahoo.com.br., Abreu DC; Department of Chemistry, Federal University of Lavras, 37200-000 Lavras, MG, Brazil. Electronic address: daianynha_cassia@hotmail.com., Saczk AA; Department of Chemistry, Federal University of Lavras, 37200-000 Lavras, MG, Brazil. Electronic address: adelir@dqi.ufla.br., Bazana MJ; Department of Chemistry, Federal University of Lavras, 37200-000 Lavras, MG, Brazil. Electronic address: matheus.julien@hotmail.com., Coelho SM; Department of Chemistry, Federal University of Lavras, 37200-000 Lavras, MG, Brazil. Electronic address: sabrina10mcoelho@yahoo.com.br., E Rosa PV; Department of Animal Science, Federal University of Lavras, 37200-000 Lavras, MG, Brazil. Electronic address: priscila@dzo.ufla.br., Magriotis ZM; Department of Chemistry, Federal University of Lavras, 37200-000 Lavras, MG, Brazil. Electronic address: zuy@dqi.ufla.br., de Lima RM; Department of Chemistry, Federal University of Lavras, 37200-000 Lavras, MG, Brazil. Electronic address: rafaelmachadolima@hotmail.com.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2017 Aug 15; Vol. 229, pp. 674-679. Date of Electronic Publication: 2017 Feb 21.
DOI: 10.1016/j.foodchem.2017.02.087
Abstrakt: The SDME-GC/MS method was applied to residual determination of anesthetic menthol in fish. The extractions took place from the headspace of the sample using 1.8µL of octane as the extraction solvent. To obtain the ideal extraction condition, was used Response Surface Methodology, defining: extraction time 15min, temperature 30°C and salt 3g. The method showed LOD and LOQ of 0.021 and 1.56µgL -1 respectively, recovery of 94% and R 2 of 0.9997. The analyzes were performed on tilapia fillets anesthetized in five concentrations between 5 and 15×10 4 µgL -1 and with times of slaughter after anesthesia of 0, 12, 24 and 48h. It was determined that 48h is the required residual period for total metabolization of menthol in the fishes' organisms. This methodology becomes promising regarding the establishment of protocols to regulatory the use of menthol as an anesthetic in aquaculture.
(Copyright © 2017 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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