Novel chitin scaffolds derived from marine sponge Ianthella basta for tissue engineering approaches based on human mesenchymal stromal cells: Biocompatibility and cryopreservation.

Autor: Mutsenko VV; Institute for Problems of Cryobiology and Cryomedicine of the NAS Ukraine, Pereyaslavskaya str. 23, 61015 Kharkov, Ukraine; Institute for Multiphase Processes, Leibniz Universität Hannover, Callinstraße 36, 30167 Hannover, Germany. Electronic address: mutsenko@imp.uni-hannover.de., Gryshkov O; Institute for Multiphase Processes, Leibniz Universität Hannover, Callinstraße 36, 30167 Hannover, Germany., Lauterboeck L; Institute for Multiphase Processes, Leibniz Universität Hannover, Callinstraße 36, 30167 Hannover, Germany., Rogulska O; Institute for Problems of Cryobiology and Cryomedicine of the NAS Ukraine, Pereyaslavskaya str. 23, 61015 Kharkov, Ukraine., Tarusin DN; Institute for Problems of Cryobiology and Cryomedicine of the NAS Ukraine, Pereyaslavskaya str. 23, 61015 Kharkov, Ukraine., Bazhenov VV; Institute of Experimental Physics, TU Bergakademie Freiberg, Leipziger Straße 23, 09599 Freiberg, Germany., Schütz K; Centre for Translational Bone, Joint and Soft Tissue Research, Faculty of Medicine and University Hospital Carl Gustav Carus of Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany., Brüggemeier S; Centre for Translational Bone, Joint and Soft Tissue Research, Faculty of Medicine and University Hospital Carl Gustav Carus of Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany., Gossla E; Centre for Translational Bone, Joint and Soft Tissue Research, Faculty of Medicine and University Hospital Carl Gustav Carus of Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany., Akkineni AR; Centre for Translational Bone, Joint and Soft Tissue Research, Faculty of Medicine and University Hospital Carl Gustav Carus of Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany., Meißner H; Centre for Translational Bone, Joint and Soft Tissue Research, Faculty of Medicine and University Hospital Carl Gustav Carus of Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany., Lode A; Centre for Translational Bone, Joint and Soft Tissue Research, Faculty of Medicine and University Hospital Carl Gustav Carus of Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany., Meschke S; BromMarin GmbH, Wernerstraße 1, 09599 Freiberg, Germany., Fromont J; Department of Aquatic Zoology, Western Australian Museum, Locked Bag 49, Welshpool DC, Western Australia 6986, Australia., Stelling AL; Department of Biochemistry, Duke University School of Medicine, Durham, NC, USA., Tabachnik KR; International Institute of Biomineralization GmbH, Talweg 26, 09599 Freiberg, Germany., Gelinsky M; Centre for Translational Bone, Joint and Soft Tissue Research, Faculty of Medicine and University Hospital Carl Gustav Carus of Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany., Nikulin S; Department of Aquatic Zoology, Western Australian Museum, Locked Bag 49, Welshpool DC, Western Australia 6986, Australia; Moscow Institute of Physics and Technology, 9 Institutskii per., 141700 Dolgoprudny, Moscow Region, Russia., Rodin S; P.A. Hertsen Moscow Research Oncology Institute, Botkinskii p.3, 125284 Moscow, Russia., Tonevitsky AG; P.A. Hertsen Moscow Research Oncology Institute, Botkinskii p.3, 125284 Moscow, Russia., Petrenko AY; Institute for Problems of Cryobiology and Cryomedicine of the NAS Ukraine, Pereyaslavskaya str. 23, 61015 Kharkov, Ukraine., Glasmacher B; Institute for Multiphase Processes, Leibniz Universität Hannover, Callinstraße 36, 30167 Hannover, Germany., Schupp PJ; Institute for Chemistry and Biology of the Marine Environment, University of Oldenburg, Carl-von-Ossietzky-Straße 9-11, 26111 Oldenburg, Germany., Ehrlich H; Institute of Experimental Physics, TU Bergakademie Freiberg, Leipziger Straße 23, 09599 Freiberg, Germany. Electronic address: Hermann.Ehrlich@physik.tu-freiberg.de.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2017 Nov; Vol. 104 (Pt B), pp. 1955-1965. Date of Electronic Publication: 2017 Mar 30.
DOI: 10.1016/j.ijbiomac.2017.03.161
Abstrakt: The extraordinary biocompatibility and mechanical properties of chitinous scaffolds from marine sponges endows these structures with unique properties that render them ideal for diverse biomedical applications. In the present work, a technological route to produce "ready-to-use" tissue-engineered products based on poriferan chitin is comprehensively investigated for the first time. Three key stages included isolation of scaffolds from the marine demosponge Ianthella basta, confirmation of their biocompatibility with human mesenchymal stromal cells, and cryopreservation of the tissue-like structures grown within these scaffolds using a slow cooling protocol. Biocompatibility of the macroporous, flat chitin scaffolds has been confirmed by cell attachment, high cell viability and the ability to differentiate into the adipogenic lineage. The viability of cells cryopreserved on chitin scaffolds was reduced by about 30% as compared to cells cryopreserved in suspension. However, the surviving cells were able to retain their differentiation potential; and this is demonstrated for the adipogenic lineage. The results suggest that chitin from the marine demosponge I. basta is a promising, highly biocompatible biomaterial for stem cell-based tissue-engineering applications.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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