Adipose Stem Cell Function Maintained with Age: An Intra-Subject Study of Long-Term Cryopreserved Cells.
Autor: | Kokai LE; Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA, USA., Traktuev DO; Division of Cardiology, Indiana School of Medicine, Indiana University, Indianapolis, IN, USA., Zhang L; Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA, USA., Merfeld-Clauss S; Division of Cardiology, Indiana School of Medicine, Indiana University, Indianapolis, IN, USA., DiBernardo G; Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA, USA., Lu H; Division of Cardiology, Indiana School of Medicine, Indiana University, Indianapolis, IN, USA., Marra KG; Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA, USA., Donnenberg A; Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Donnenberg V; Department of Cardiothoracic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Meyer EM; Flow Cytometry Facility, University of Pittsburgh Cancer Center, Pittsburgh, PA, USA., Fodor PB; Plastic surgeon in private practice in Santa Monica, CA, USA., March KL; Division of Cardiology, Indiana School of Medicine, Indiana University, Indianapolis, IN, USA., Rubin JP; Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA, USA. |
---|---|
Jazyk: | angličtina |
Zdroj: | Aesthetic surgery journal [Aesthet Surg J] 2017 Apr 01; Vol. 37 (4), pp. 454-463. |
DOI: | 10.1093/asj/sjw197 |
Abstrakt: | Background: The progressive decline in tissue mechanical strength that occurs with aging is hypothesized to be due to a loss of resident stem cell number and function. As such, there is concern regarding use of autologous adult stem cell therapy in older patients. To abrogate this, many patients elect to cryopreserve the adipose stromal-vascular fraction (SVF) of lipoaspirate, which contains resident adipose stem cells (ASC). However, it is not clear yet if there is any clinical benefit from banking cells at a younger age. Objectives: We performed a comparative analysis of SVF composition and ASC function from cells obtained under GMP conditions from the same three patients with time gap of 7 to 12 years. Methods: SVF, cryobanked under good manufacturing practice (GMP) conditions, was thawed and cell yield, viability, and cellular composition were assessed. In parallel, ASC proliferation and efficiency of tri-lineage differentiation were evaluated. Results: The results showed no significant differences existed in cell yield and SVF subpopulation composition within the same patient between harvest procedures 7 to 12 years apart. Further, no change in proliferation rates of cultured ASCs was found, and expanded cells from all patients were capable of tri-lineage differentiation. Conclusions: By harvesting fat from the same patient at two time points, we have shown that despite the natural human aging process, the prevalence and functional activity of ASCs in an adult mesenchymal stem cell, is highly preserved. Level of Evidence: 5. (© 2016 The American Society for Aesthetic Plastic Surgery, Inc. Reprints and permission: journals.permissions@oup.com) |
Databáze: | MEDLINE |
Externí odkaz: |