| Autor: |
Cui TZ; Department of Cell Biology, Yale School of Medicine, New Haven, United States., Peterson TA; Department of Molecular Physiology and Biophysics, University of Iowa, Iowa City, United States., Burd CG; Department of Cell Biology, Yale School of Medicine, New Haven, United States. |
| Jazyk: |
angličtina |
| Zdroj: |
ELife [Elife] 2017 Mar 31; Vol. 6. Date of Electronic Publication: 2017 Mar 31. |
| DOI: |
10.7554/eLife.24126 |
| Abstrakt: |
We describe a regulatory mechanism that controls the activity of retromer, an evolutionarily conserved sorting device that orchestrates cargo export from the endosome. A spontaneously arising mutation that activates the yeast ( Saccharomyces cerevisiae ) CDC25 family phosphatase, Mih1, results in accelerated turnover of a subset of endocytosed plasma membrane proteins due to deficient sorting into a retromer-mediated recycling pathway. Mih1 directly modulates the phosphorylation state of the Vps26 retromer subunit; mutations engineered to mimic these states modulate the binding affinities of Vps26 for a retromer cargo, resulting in corresponding changes in cargo sorting at the endosome. The results suggest that a phosphorylation-based gating mechanism controls cargo selection by yeast retromer, and they establish a functional precedent for CDC25 protein phosphatases that lies outside of their canonical role in regulating cell cycle progression. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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