Assessment of pharmacokinetics for microvessel proliferation by DCE-MRI for early detection of physeal bone bridge formation in an animal model.

Autor: Neumayer B; Ludwig Boltzmann Institute for Clinical Forensic Imaging, Universitätsplatz 4, 8010, Graz, Austria.; BioTechMed, University of Graz, Universitaetsplatz 3, 8010, Graz, Austria., Amerstorfer E; Department of Paediatric and Adolescent Surgery, Medical University of Graz, Auenbruggerplatz 34, 8036, Graz, Austria., Diwoky C; BioTechMed, University of Graz, Universitaetsplatz 3, 8010, Graz, Austria.; Institute of Molecular Biosciences, University of Graz, Humboldtstraße 50, 8010, Graz, Austria., Lindtner RA; Department of Trauma Surgery, Medical University of Innsbruck, Anichstrasse 35, 6020, Innsbruck, Austria., Wadl E; Department of Pathology, Clinical Center Klagenfurt, Feschnigstraße 11, 9020, Klagenfurt, Austria., Scheurer E; Institute of Forensic Medicine, University of Basel, Pestalozzistraße 22, 4056, Basel, Switzerland., Weinberg AM; Department of Orthopedics and Orthopedic Surgery, Medical University of Graz, Auenbruggerplatz 5, 8036, Graz, Austria., Stollberger R; BioTechMed, University of Graz, Universitaetsplatz 3, 8010, Graz, Austria. rudolf.stollberger@tugraz.at.; Institute of Medical Engineering, Graz University of Technology, Stremayrgasse 16/III, 8010, Graz, Austria. rudolf.stollberger@tugraz.at.
Jazyk: angličtina
Zdroj: Magma (New York, N.Y.) [MAGMA] 2017 Oct; Vol. 30 (5), pp. 417-427. Date of Electronic Publication: 2017 Mar 30.
DOI: 10.1007/s10334-017-0615-2
Abstrakt: Objectives: Bone bridge formation occurs after physeal lesions and can lead to growth arrest if not reversed. Previous investigations on the underlying mechanisms of this formation used histological methods. Therefore, this study aimed to apply a minimally invasive method using dynamic contrast-enhanced MRI (DCE-MRI).
Materials and Methods: Changes in functional parameters related to the microvessel system were assessed in a longitudinal study of a cohort of an animal model applying a reference region model. The development of morphology of the injured physis was investigated with 3D high-resolution MRI. To acquire complementary information for MRI-related findings qRT-PCR and immunohistochemical data were acquired for a second cohort of the animal model.
Results: The evaluation of the pharmacokinetic parameters showed a first rise of the transfer coefficient 7 days post-lesion and a maximum 42 days after operation. The analysis of the complementary data showed a connection of the first rise to microvessel proliferation while the maximum value was linked to bone remodeling.
Conclusion: The pharmacokinetic analysis of DCE-MRI provides information on a proliferation of microvessels during the healing process as a sign for bone bridge formation. Thereby, DCE-MRI could identify details, which up to now required analyses of highly invasive methods.
Databáze: MEDLINE
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