| Autor: |
Young JM; Molecular Biology Division, Rorer Biotechnology, Inc., Springfield, VA 22151., Cheadle C, Foulke JS Jr, Drohan WN, Sarver N |
| Jazyk: |
angličtina |
| Zdroj: |
Gene [Gene] 1988; Vol. 62 (2), pp. 171-85. |
| DOI: |
10.1016/0378-1119(88)90556-2 |
| Abstrakt: |
Epstein-Barr virus (EBV) replicons which include the genetic element oriP and a functional gene for Epstein-Barr nuclear antigen (EBNA-1) can be maintained episomally in a variety of mammalian cell lines [Yates et al., Nature 313 (1985) 812-815]. We have assessed the application of an EBV replicon for foreign gene expression. Two cDNAs, human interferon-gamma (IFN-gamma) and the extracellular domain of the human epidermal growth factor receptor (EGF-Rex), cloned in an EBV replicon, were efficiently expressed and the protein was secreted into the extracellular media. Expression in human embryonic 293 cells was approximately ten-fold higher than in CV-1 cells. The expression of the human protein is dependent upon the orientation of the IFN-gamma transcriptional cassette relative to the other genetic elements within the vector. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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