Involvement of oxidative stress in protocatechuic acid-mediated bacterial lethality.

Autor: Ajiboye TO; Antioxidants, Redox Biology and Toxicology Research Laboratory, Department of Biological Sciences, Al-Hikmah University, Ilorin, Nigeria., Habibu RS; Antioxidants, Redox Biology and Toxicology Research Laboratory, Department of Biological Sciences, Al-Hikmah University, Ilorin, Nigeria., Saidu K; Antioxidants, Redox Biology and Toxicology Research Laboratory, Department of Biological Sciences, Al-Hikmah University, Ilorin, Nigeria., Haliru FZ; Antioxidants, Redox Biology and Toxicology Research Laboratory, Department of Biological Sciences, Al-Hikmah University, Ilorin, Nigeria., Ajiboye HO; Department of Biochemistry, University of Ilorin, Ilorin, Nigeria., Aliyu NO; Department of Biochemistry, University of Ilorin, Ilorin, Nigeria., Ibitoye OB; Antioxidants, Redox Biology and Toxicology Research Laboratory, Department of Biological Sciences, Al-Hikmah University, Ilorin, Nigeria., Uwazie JN; Department of Biochemistry, University of Ilorin, Ilorin, Nigeria., Muritala HF; Department of Biochemistry, University of Ilorin, Ilorin, Nigeria., Bello SA; Microbiology Unit, Department of Biological Sciences, Al-Hikmah University, Ilorin, Nigeria., Yusuf II; Microbiology Unit, Department of Biological Sciences, Al-Hikmah University, Ilorin, Nigeria., Mohammed AO; Microbiology Unit, Department of Biological Sciences, Al-Hikmah University, Ilorin, Nigeria.
Jazyk: angličtina
Zdroj: MicrobiologyOpen [Microbiologyopen] 2017 Aug; Vol. 6 (4). Date of Electronic Publication: 2017 Mar 27.
DOI: 10.1002/mbo3.472
Abstrakt: The involvement of oxidative stress in protocatechuic acid-mediated bacterial lethality was investigated. Minimum inhibitory concentrations (MIC) and minimum bactericidal concentration (MBC) of protocatechuic acid against Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus are 600 and 700 μg/ml, 600 and 800 μg/ml, and 600 and 800 μg/ml, respectively. The optical densities and colony-forming units of protocatechuic acid-treated bacteria decreased in time-dependent manner. Protocatechuic acid (4× MIC) significantly increased the superoxide anion content of E. coli, P. aeruginosa, and S. aureus compared to dimethyl sulfoxide (DMSO). Superoxide dismutase, catalase, and NAD + /NADH in protocatechuic acid-treated E. coli, P. aeruginosa, and S. aureus increased significantly when compared to DMSO. Conversely, level of reduced glutathione decreased in protocatechuic acid-treated E. coli, P. aeruginosa, and S. aureus, while glutathione disulfide increased when compared to DMSO. Furthermore, malondialdehyde and fragmented DNA increased significantly following exposure to protocatechuic acid. Protocatechuic acid inhibited the activity of complexes I and II. From the above findings, protocatechuic acid enhanced the generation of reactive oxygen species (superoxide anion radical and hydroxyl radical) in E. coli, P. aeruginosa, and S. aureus, possibly by autoxidation, fenton chemistry, and inhibiting electron transport chain resulting in lipid peroxidation and DNA fragmentation and consequentially bacterial cell death.
(© 2017 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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